[KO Validated] Anti-SEC61B Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P60468

Core Product Specifications and Parameters

Biological Background: SEC61B Function and Localization

• Protein transport protein Sec61 subunit beta (SEC61B) is a core component of the SEC61 channel-forming translocon complex located at the endoplasmic reticulum (ER) membrane. Related references: PMID:12475939
• The SEC61 complex functions as both a ribosome receptor and a gated pore, enabling cotranslational translocation of signal peptide-containing nascent polypeptides into the ER lumen. Related references: PMID:12475939
• SEC61B also participates in the ER membrane insertion of transmembrane proteins, mediating the insertion of initial transmembrane segments, while subsequent segments utilize the multi-pass translocon (MPT) complex. Related references: PMID:32820719, PMID:36261522
• Cooperates with the translocating protein TRAM1 to facilitate import of nascent proteins into the ER. Related references: PMID:19121997
• Subcellular localization is restricted to the ER membrane, consistent with its role in protein translocation.
• Post-translational modifications include acetylation, palmitoylation, and phosphorylation, which may regulate function or membrane association.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-96) of human SEC61B; the antibody is expected to detect both unmodified and modified forms near the N-terminus.
• For Western blot, SEC61B migrates at approximately 10 kDa; consider using high-percentage gels (e.g., 12-15%) and optimized transfer conditions (e.g., low molecular weight membranes, short transfer times).
• For immunofluorescence/ICC, ER-like staining pattern is anticipated; include permeabilization steps (e.g., 0.1% Triton X-100) to enable antibody access to the membrane protein.
• ELISA applications may employ recombinant SEC61B fragments or peptide antigens; confirm coating efficiency and antibody specificity with appropriate controls.
• As a polyclonal antibody, batch-to-batch consistency should be validated; use knockout cell lines or peptide blocking assays to confirm target specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibodies, antigens, and ancillary reagents for protein translocation and ER biology research. For diagnostic developers seeking to integrate SEC61B detection into assays, we supply optimized polyclonal and monoclonal antibodies alongside bulk reagent options. Our global sourcing network ensures consistent access to rare-target raw materials, complemented by technical consulting to accelerate your product development pipeline.