[KO Validated] Anti-SCYL1 Rabbit Polyclonal Antibody for WB, ELISA - Q96KG9

Core Product Specifications and Parameters

Biological Background: SCYL1 Function and Localization

• Also known as N-terminal kinase-like protein, coated vesicle-associated kinase of 90 kDa (CVAK90), NTKL, TAPK, TEIF, and TRAP; encoded by the SCYL1 gene in humans.
• Regulates COPI-mediated retrograde protein traffic at the Golgi–ER interface and is required for the maintenance of Golgi apparatus morphology. Related references: PMID:18556652 PMID:26581903
• Acts as a transcriptional activator by binding to GC-rich DNA motifs in the promoter regions of beta-polymerase and TERT.
• Localizes to multiple subcellular compartments including the cytoplasm, centrosome, ER-Golgi intermediate compartment, cis-Golgi network, and nucleus.
• Ubiquitously expressed across human tissues.
• Annotated as a phosphoprotein and contains coiled-coil domains; disease associations include spinocerebellar ataxia, intellectual disability, and neurodegeneration.

Experimental Guidance and Technical Tips

• The immunogen (aa 460-740) covers the C‑terminal region of SCYL1; it is suitable for detecting full‑length protein (~90 kDa) in Western blot.
• As a KO‑validated antibody, it has demonstrated target specificity; nevertheless, confirm suitability in your specific experimental context.
• For WB, consider using lysates from human (HeLa, HEK293), mouse, or rat cell lines/tissues, given the confirmed cross‑reactivity.
• For ELISA, the polyclonal format may provide robust signal; pair with an appropriate capture antibody and validate with a recombinant SCYL1 standard.
• Optimize antibody dilution and incubation conditions empirically for each application.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined access to high‑quality IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare‑target sourcing. For SCYL1‑related studies in intracellular trafficking and transcription, we can help secure the right reagents to accelerate your assay development and clinical translation.