[KO Validated] Anti-SAMHD1 Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - Q9Y3Z3

Core Product Specifications and Parameters

Biological Background: SAMHD1 Function and Localization

• SAMHD1 (Deoxynucleoside triphosphate triphosphohydrolase SAMHD1) is also known as Dendritic cell-derived IFNG-induced protein (DCIP), Monocyte protein 5 (MOP-5), and SAM domain and HD domain-containing protein 1 (hSAMHD1).
• Possesses dNTPase activity that reduces cellular dNTP pools, restricting HIV-1 replication in dendritic and other myeloid cells by limiting reverse transcription. Related references: PMID:19525956 PMID:21613998 PMID:21720370
• Restriction activity is counteracted by HIV-2 viral protein Vpx, which targets SAMHD1 for degradation. Related references: PMID:21613998 PMID:21720370
• Phosphorylation at Thr-592 acts as a molecular switch: it inhibits dNTPase/restriction function while promoting DNA end resection at stalled replication forks. Related references: PMID:23601106 PMID:23602554 PMID:29610582
• Stimulates the exonuclease activity of MRE11 at stalled forks, activating the ATR-CHK1 pathway to enable replication restart. Related references: PMID:29670289
• Prevents type I interferon induction and chronic inflammation by promoting degradation of nascent DNA at stalled replication forks. Related references: PMID:27477283 PMID:29670289
• Expressed in heart, skeletal muscle, spleen, liver, small intestine, placenta, lung, and peripheral blood leukocytes; not detected in brain or thymus. Related references: PMID:11064105
• Involved in antiviral defense, DNA repair, and dNTP pool regulation; also enhances immunoglobulin hypermutation in B-lymphocytes.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 387–626, covering evolutionarily conserved regions likely to recognize native SAMHD1 across human, mouse, and rat samples.
• For western blot, a band at approximately 72 kDa is expected; use appropriate positive and negative cell lysates to confirm specificity.
• In IHC-P, consider antigen retrieval methods and titration to optimize staining; inclusion of KO tissue controls enhances confidence.
• The polyclonal nature offers robust signal but may exhibit lot-to-lot variability; validate each new lot in your assay system.
• For IP and ELISA, follow standard protocols and include negative controls to account for non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined path to essential IVD raw materials, complemented by technical and consulting support from concept to clinic. This anti-SAMHD1 polyclonal antibody supports research in antiviral defense and genome stability, and we can further supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, or assist in sourcing rare target raw materials for your assay development needs.