KO Validated Anti-RPL22 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P35268

Core Product Specifications and Parameters

Biological Background: RPL22 Function and Localization

• RPL22 encodes the 60S ribosomal protein L22 (eL22), an essential component of the large ribosomal subunit.
• RPL22 is a component of the large ribosomal subunit, which is a ribonucleoprotein complex responsible for cellular protein synthesis. Related references: PMID:23636399 PMID:32669547
• Alternative names include EBER-associated protein (EAP), reflecting its binding to Epstein-Barr virus small RNA, and heparin-binding protein HBp15, indicating additional RNA- and heparin-binding capabilities.
• Subcellularly, RPL22 localizes primarily in the cytoplasm, consistent with its role in cytosolic translation.
• Post-translational modifications such as phosphorylation have been annotated, suggesting regulatory potential.
• The RPL22 gene family includes conserved isoforms; this antibody targets the N-terminal region (amino acids 1–122), which may recognize multiple isoforms if the epitope is preserved.

Experimental Guidance and Technical Tips

• KO validation confirms the antibody's specificity; always include appropriate positive and negative cell/tissue controls in your experiments.
• For Western blot, a band around 15 kDa is expected; consider loading controls and molecular weight markers for accurate size determination.
• IHC-P may require optimization of antigen retrieval and primary antibody dilution; validate with known positive and negative tissue sections.
• The immunogen (N-terminal 1–122 aa) suggests the antibody may recognize both native and denatured RPL22; in ELISA, test coating conditions accordingly.
• Cross-reactivity with mouse and rat indicates conserved epitopes, but individual assay validation is recommended for each species.

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As a one-stop IVD raw-material partner, CamelBio supports diagnostic developers and researchers with high-quality reagents like this anti-RPL22 polyclonal antibody for ribosome and translation studies. Our extensive portfolio covers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—helping you move from discovery to clinical application efficiently. Contact us to discuss how we can accelerate your next project with reliable, curated raw materials.