[KO Validated] Anti-RhoGDI Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P52565

Core Product Specifications and Parameters

Biological Background: RhoGDI Function and Localization

• Rho GDP-dissociation inhibitor 1 (RhoGDI, also known as Rho-GDI alpha) is encoded by the ARHGDIA (GDIA1) gene and is a key regulator of Rho GTPase signaling.
• It inhibits the dissociation of GDP from Rho proteins such as CDC42, RAC1, and RHOA, thereby maintaining them in an inactive cytosolic pool and protecting them from degradation.
• RhoGDI actively participates in the recycling and distribution of activated Rho GTPases; its exceptionally high affinity for prenylated forms allows it to extract both inactive and activated molecules from membranes.
• Through modulation of Rho proteins, RhoGDI may regulate cell motility; in glioma cells, it inhibits migration and invasion by mediating SEMA5A/PLXNB3 signals that inactivate RAC1.
• Subcellular localization is predominantly cytoplasmic.
• Post-translational modifications include acetylation and phosphorylation; alternative splicing produces multiple isoforms, and the protein is subject to ubiquitination.
• Disease-associated variants of ARHGDIA are linked to nephrotic syndrome type 8 (NPHS8), highlighting its physiological importance.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length RhoGDI protein (amino acids 1–204), so this polyclonal antibody likely recognizes multiple epitopes. Use appropriate positive and negative controls in all applications.
• In Western blot, a specific band at ~23 kDa is expected. Confirm reactivity by using recombinant protein or cell lines with known overexpression or knockout.
• For immunofluorescence/ICC, optimize fixation and permeabilization conditions due to cytoplasmic localization. Include isotype controls to assess background.
• ELISA can be used for quantitative detection; verify cross-reactivity with other RhoGDI family members if assay specificity is critical.
• The antibody is KO-validated, but it is advisable to independently confirm specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our RhoGDI polyclonal antibody supports investigations into Rho signaling and cell motility, critical in cancer and kidney disease research. We offer validated antibody pairs, optimized polyclonal/monoclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development.