[KO Validated] Anti-RhoGDI Rabbit Monoclonal Antibody for WB and ELISA - P52565

Core Product Specifications and Parameters

Biological Background: RhoGDI (Rho GDP-dissociation inhibitor 1) Function and Localization

• RhoGDI (Rho GDP-dissociation inhibitor 1, also known as Rho-GDI alpha, GDIA1, NPHS8, RHOGDI-1) is encoded by the ARHGDIA gene (Gene ID: 396). It functions as a key regulator of Rho protein homeostasis by controlling the GDP/GTP exchange reaction.
• By inhibiting the dissociation of GDP from Rho proteins, RhoGDI prevents GTP binding, thereby maintaining small GTPases such as CDC42, RAC1, and RHOA in an inactive cytosolic pool and protecting them from degradation.
• RhoGDI exhibits exceptionally high affinity for prenylated Rho proteins, allowing it to extract both inactive and activated forms from cellular membranes and regulate their recycling and subcellular distribution.
• Through its modulation of Rho GTPase activity, RhoGDI influences cell motility; in glioma cells, it inhibits migration and invasion by mediating SEMA5A and PLXNB3 signals that inactivate RAC1.
• The protein is predominantly localized to the cytoplasm, consistent with its role in sequestering Rho GTPases away from membrane-bound activation sites.
• Post-translational modifications affecting RhoGDI include acetylation and phosphorylation, and it is a target for ubiquitin-like conjugation, potentially modulating its stability and function.
• The canonical isoform has a predicted molecular weight of approximately 23 kDa (calculated from sequence: ~23.2 kDa). Alternative splicing gives rise to additional isoforms, and the protein is a reference proteome member.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 70-150 of human RhoGDI, a region conserved across human, mouse, and rat, which supports the observed cross-reactivity.
• For Western blotting, a band at approximately 23 kDa is expected; consider using a low-molecular-weight range gel (e.g., 12-15% acrylamide) for optimal resolution.
• This antibody can be used in ELISA; appropriate sample preparation and optimization (e.g., antigen coating concentration, blocking conditions) are recommended for reliable results.
• Since RhoGDI is a cytoplasmic protein, cell lysates should be prepared with appropriate lysis buffers to ensure efficient extraction and avoid degradation of the target.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, contract research organizations, and academic laboratories with streamlined one-stop access to high-quality IVD raw materials. This anti-RhoGDI monoclonal antibody complements our portfolio for Rho GTPase signaling research. Beyond individual antibodies, we offer validated antibody pairs, optimized recombinant proteins, bulk ancillary reagents, and customized sourcing for rare targets to support your entire development pipeline.