[KO Validated] Anti-RELA Rabbit Polyclonal Antibody for WB, ELISA - Q04207

Core Product Specifications and Parameters

Biological Background: RELA Function and Localization

• RELA, also known as transcription factor p65 or nuclear factor NF-kappa-B p65 subunit, is a member of the NF-kB/Rel protein family.
• NF-kB is a pleiotropic transcription factor that regulates biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis, and apoptosis.
• RELA forms homo- or heterodimers with other Rel-like domain-containing proteins; the RELA-NFKB1 heterodimer is the most abundant and functions as a transcriptional activator.
• NF-kB dimers are retained in the cytoplasm in an inactive state by binding to IkB inhibitors; upon stimulation, IkB is phosphorylated and degraded, allowing NF-kB to translocate to the nucleus.
• Subcellular localization is dynamic: predominantly cytoplasmic in resting cells and nuclear upon activation; it shuttles between the nucleus and cytoplasm.
• Post-translational modifications, including acetylation, phosphorylation, and methylation, modulate its transcriptional activity and interactions.
• RELA can also modulate promoter accessibility to transcription factors, indirectly regulating gene expression (PubMed:29813070). Related references: PMID:29813070
• At the protein level, RELA is expressed in the myocardium, among other tissues.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering mouse RELA; the antibody is polyclonal and validated by knockout (KO).
• For Western blot, expect a band at ~60 kDa. Use appropriate positive controls (e.g., mouse tissue lysates known to express RELA) and validate specificity in your sample system.
• For ELISA, consider checkerboard titration to optimize coating and detection antibody concentrations; cross-reactivity with human allows potential use in human samples after validation.
• Due to the dynamic regulation of RELA, consider using both cytoplasmic and nuclear fractionation to assess activation status.
• Always include negative controls (e.g., knockout cell lines or tissues) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For NF-kB pathway research, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your immunoassay development. Contact us to discuss how we can bridge your supply needs from discovery to clinical application.