[KO Validated] Anti-RBM3 Polyclonal Antibody for WB, IF/ICC, ELISA - P98179

Core Product Specifications and Parameters

Biological Background: RBM3 Function and Localization

• RBM3 (RNA-binding protein 3, also known as RNPL or IS1-RNPL) is a cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures.
• It reduces the relative abundance of microRNAs when overexpressed, thereby contributing to translational regulation.
• RBM3 enhances the phosphorylation of translation initiation factors and promotes active polysome formation, facilitating efficient translation.
• It localizes to the nucleus, cytoplasm, and cell projections such as dendrites, suggesting roles in mRNA transport and local protein synthesis.
• Post-translational modifications include methylation and phosphorylation, which may regulate its activity.
• Keywords associated with RBM3 include stress response, RNA-binding, and proteomics identification, highlighting its role in cellular stress adaptation.

Experimental Guidance and Technical Tips

• This antibody was generated against a recombinant fusion protein covering amino acids 1–157 of human RBM3; ensure the immunogen sequence is conserved in your target species.
• For Western blotting (WB), RBM3 has a predicted molecular weight of ~17 kDa; consider using appropriate gel percentage and transfer conditions for low molecular weight proteins.
• In immunofluorescence/immunocytochemistry (IF/ICC), RBM3 can be detected in the nucleus and cytoplasm; validate subcellular localization in your cell model.
• ELISA applications may require optimization of coating antigen and antibody concentration.
• Cross-reactivity with mouse and rat has been reported; verify detection in your specific sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This RBM3 antibody is ideal for investigations into cold-inducible translational regulation and stress response pathways. Contact us to discuss your procurement needs and technical support requirements.