[KO Validated] Anti-Rad23A Rabbit Polyclonal Antibody for WB, ELISA - P54725

Core Product Specifications and Parameters

Biological Background: Rad23A Function and Localization

• Rad23A, also known as HR23A or hHR23A, is a multifunctional protein that is also referred to as lysine-specific demethylase RAD23A.
• Functions in histone H4K20 demethylation, removing mono-, di-, and trimethyl marks to produce unmethylated H4K20, thereby regulating transcriptional activation of coding genes and repetitive elements. Related references: PMID:32209475
• Participates in nucleotide excision repair (NER) by forming a complex with XPC; the XPC-RAD23A complex is critical for global genome NER and can stabilize XPC. Related references: PMID:9372924
• Involved in ubiquitin-dependent protein degradation by binding to 'Lys-48'-linked polyubiquitin chains and delivering ubiquitinated substrates to the 26S proteasome. Related references: PMID:12643283 PMID:14621999 PMID:15321727
• Plays a role in HIV-1 replication by facilitating the association of HIV-1 Vpr with the host proteasome in non-proliferating cells and primary macrophages.
• Localizes predominantly to the nucleus, consistent with its functions in DNA repair, chromatin modification, and transcriptional regulation.
• Contains ubiquitin-like (UBL) domains and belongs to the UBL family; keywords include Ubl conjugation, repeat, and phosphoprotein.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was generated against a recombinant protein encompassing amino acids 1-363 of human Rad23A, which includes N-terminal ubiquitin-like domains and functional regions. Consider validating specificity using knockout (KO) cell lines or tissues, in line with the antibody's KO-validated status.
• For Western blot analysis, a band at approximately 40 kDa is expected. Due to potential post-translational modifications or isoform expression, confirm the observed molecular weight in your specific sample system.
• The antibody is suitable for ELISA applications; optimize coating conditions and antibody dilutions for your assay format.
• Cross-reactivity has been demonstrated with human and mouse samples; if using other species, perform appropriate verification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated one-stop sourcing platform for IVD raw materials, empowering diagnostic manufacturers and research laboratories to accelerate development from concept to clinic. For DNA repair and chromatin biology research areas, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies such as this anti-Rad23A product, and bulk ancillary reagents. Our comprehensive offerings support your entire workflow, ensuring reliable access to high-quality raw materials and technical consultation.