[KO Validated] Anti-PTBP1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P26599

Core Product Specifications and Parameters

Biological Background: PTBP1 Function and Localization

• PTBP1, also known as heterogeneous nuclear ribonucleoprotein I (hnRNP I), is a 57 kDa RNA-binding protein encoded by the PTBP1 gene in humans.
• It plays a key role in pre-mRNA splicing and regulates alternative splicing by binding to polypyrimidine tracts in introns, often promoting exon skipping.
• PTBP1 represses the splicing of MAPT/Tau exon 10. Related references: PMID:15009664
• It binds to the polypyrimidine-rich controlling element (PCE) of CFTR and promotes exon 9 skipping, thereby antagonizing TIA1. Related references: PMID:14966131
• In pyruvate kinase PKM splicing, PTBP1 binds repressively to a polypyrimidine tract flanking exon 9, leading to PKM M2 isoform production. Related references: PMID:20010808
• Upon picornavirus infection, PTBP1 binds to the viral internal ribosome entry site (IRES) and stimulates IRES-mediated translation. Related references: PMID:21518806
• PTBP1 is predominantly localized in the nucleus and undergoes multiple post‑translational modifications, including acetylation, phosphorylation, isopeptide bond formation, and ubiquitin‑like conjugation.
• It contains RNA recognition motifs (RRMs) and is involved in RNA looping and snRNP recruitment during spliceosome assembly.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–290 of human PTBP1, covering the N‑terminal region; consider this when assessing antibody specificity in your assay system.
• PTBP1 migrates at approximately 60 kDa in reducing SDS‑PAGE; verify the observed band against appropriate molecular weight markers and known controls in western blot.
• For immunohistochemistry (IHC‑P) and immunofluorescence (IF/ICC), a nuclear signal is expected; use appropriate counterstains to confirm localization.
• Cross‑reactivity with mouse and rat PTBP1 supports use in rodent models; validate performance in your target tissue or cell line.
• ELISA is listed as a compatible application; optimize coating and detection conditions for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

As a one‑stop IVD raw‑material sourcing partner, CamelBio provides validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Our PTBP1‑related offerings support alternative splicing research and diagnostic assay development. From concept to clinic, CamelBio streamlines raw‑material procurement for laboratories and manufacturers worldwide.