KO Validated Anti-PSME2 Rabbit Polyclonal Antibody for WB and ELISA - Q9UL46

Core Product Specifications and Parameters

Biological Background: PSME2 Function and Localization

• PSME2 encodes the beta subunit of the 11S regulator complex (PA28), also known as PA28beta, REGbeta, or activator of multicatalytic protease subunit 2.
• The PA28 activator complex binds to the 20S proteasome and alters its cleavage pattern, enhancing the generation of class I MHC-binding peptides for efficient antigen processing.
• PSME2 is implicated in immunoproteasome assembly, which is crucial for adaptive immune responses.
• It is a phosphoprotein and undergoes acetylation, as identified by proteomics studies, suggesting dynamic regulation by post-translational modifications.
• The calculated molecular weight of PSME2 is approximately 27.4 kDa, consistent with its observed size of 27 kDa in experimental conditions.
• Keyword annotations include 3D-structure, direct protein sequencing, and reference proteome, highlighting its well-characterized nature.

Experimental Guidance and Technical Tips

• The provided KO validation suggests high specificity; consider using PSME2 knockout cell lysates as a negative control in WB to confirm band identity.
• For WB, a band around 27 kDa is expected. Optimize gel percentage and transfer conditions for low molecular weight proteins.
• This polyclonal antibody is suitable for ELISA; use recombinant PSME2 protein as a standard for quantitative assays.
• Since the antibody is polyclonal, slight lot-to-lot variability may occur; always titrate and validate in the relevant sample system.
• Cross-reactivity with mouse PSME2 enables translational studies in mouse models of immune function.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a comprehensive catalog of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-PSME2 antibody supports immunoproteasome research and antigen presentation studies. As a sourcing bridge, CamelBio also specializes in procuring rare-target antibodies and custom recombinant proteins to accelerate your assay development from concept to clinic.