[KO Validated] Anti-PSAP Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P07602

Core Product Specifications and Parameters

Biological Background: PSAP Function and Localization

• PSAP encodes prosaposin, a precursor protein that is proteolytically cleaved into four saposin peptides (saposin A, B, C, D), which act as essential cofactors for lysosomal sphingolipid degradation.
• Saposins facilitate the hydrolysis of sphingolipids by extracting lipid substrates from membranes and presenting them to their respective lysosomal enzymes.
• Prosaposin also functions as a myelinotrophic and neurotrophic factor, signaling through G protein-coupled receptors GPR37 and GPR37L1 to promote neuronal survival.
• Within the lysosome, saposin C activates glucocerebrosidase (GBA) by altering lipid bilayer properties, protecting it from proteolytic degradation; saposin D activates acid ceramidase; saposin A activates galactocerebrosidase; and saposin B facilitates sulfatide hydrolysis by arylsulfatase A.
• Predominantly localized to the lysosome and secreted into the extracellular space, consistent with its dual roles in intracellular lipid metabolism and intercellular signaling.
• Mutations in PSAP are linked to lysosomal storage diseases such as Gaucher disease, metachromatic leukodystrophy, and combined saposin deficiencies, as well as Parkinson’s disease.

Experimental Guidance and Technical Tips

• For Western blot, the antibody detects endogenous prosaposin at approximately 58 kDa; validate with appropriate controls such as PSAP knockout lysates.
• In immunohistochemistry, ensure antigen retrieval methods are optimized for the tissue type and consider the lysosomal/perinuclear staining pattern.
• For immunofluorescence, include a permeabilization step to allow access to intracellular lysosomal compartments; co-stain with lysosomal markers to confirm localization.
• The immunogen spans amino acids 275-524, which includes multiple saposin domains; the polyclonal nature may result in detection of both precursor and processed forms—validate specificity in your model system.
• Cross-reactivity with mouse and rat is reported; verify performance across species with positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs streamlined access to a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For neurodegeneration and lysosomal storage disease research, we support rare target sourcing and can provide tailored technical consultation from concept to clinic. Partner with us to secure high-quality reagents like this anti-PSAP antibody and accelerate your assay development.