KO Validated Anti-PRMT4/CARM1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q86X55

Core Product Specifications and Parameters

Biological Background: PRMT4/CARM1 Function and Localization

• PRMT4/CARM1 is a histone-arginine methyltransferase that catalyzes mono- and asymmetric dimethylation of arginine residues in key proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability.
• Recruited to gene promoters with histone acetyltransferases (e.g., EP300, p160 family), CARM1 methylates histone H3 at Arg-17 (H3R17me2a), leading to chromatin remodeling and transcriptional activation. Related references: PMID:12237300 PMID:16497732 PMID:19405910
• As a member of the protein arginine N-methyltransferase family, CARM1 acts as a coactivator for nuclear receptors (e.g., PPARG), transcription factors (e.g., MEF2C, NF-kappa-B, p53/TP53, TCF7L2/TCF4), and metabolic regulators (e.g., ACACA), often working synergistically with acetyltransferases.
• CARM1 localizes to the nucleus, cytoplasm, and chromosomes, dynamically shuttling to execute both nuclear (transcriptional) and cytoplasmic (post-transcriptional) regulatory functions.
• The protein is subject to acetylation, phosphorylation, and ubiquitination, and it methylates non-histone substrates including EP300, PABPC1, ELAVL1, and ELAV4, thereby modulating protein interactions and RNA stability. Related references: PMID:15731352
• CARM1 also plays a methylation-independent role in replication fork progression by promoting PARP1 recruitment and poly-ADP-ribosylation of chromatin at forks. Related references: PMID:33412112
• Tissue specificity: CARM1 is overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia, highlighting its relevance in cancer biology.
• Keywords: Chromatin regulator, methyltransferase, transcription regulation, alternative splicing, host-virus interaction, and others indicate a multifunctional protein involved in essential cellular processes.

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was raised against a recombinant fragment corresponding to amino acids 486-603 of human PRMT4/CARM1 (NP_954592.1). Researchers may consider aligning this region across species or isoforms if studying non-human models or splice variants.
• Western blotting (WB): For WB, a band at ~66 kDa is expected. Consider using nuclear-enriched lysates from human, mouse, or rat cells, and optimize antibody dilution and blocking conditions.
• Immunohistochemistry (IHC-P): For paraffin-embedded tissues, standard antigen retrieval and titration are recommended. Given CARM1 overexpression in prostate cancer, relevant tissue arrays may serve as positive controls.
• ELISA: This antibody can be used in ELISA-based assays; pair with an appropriate enzyme-conjugated secondary antibody and validate in the target sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers, laboratories, and research institutes with streamlined access to validated IVD antibodies like this anti-PRMT4/CARM1 polyclonal antibody, alongside a full range of ancillary reagents, antibody pairs, and custom rare-target development services. With expertise spanning every stage from concept to clinic, we help you source high-quality raw materials for chromatin, transcription, and oncology research applications. Contact us to discuss your bulk or specialty procurement needs.