[KO Validated] Anti-PRMT4/CARM1 Rabbit Monoclonal Antibody for WB, IP, ELISA - Q86X55

Core Product Specifications and Parameters

Biological Background: PRMT4/CARM1 Function and Localization

• CARM1 asymmetrically dimethylates histone H3 at Arg-17 (H3R17me2a), facilitating chromatin remodeling and transcriptional activation upon recruitment with histone acetyltransferases (PubMed:12237300, 16497732, 19405910). Related references: PMID:12237300 PMID:16497732 PMID:19405910
• It functions as a transcriptional coactivator for nuclear hormone receptors, TCF/β-catenin, MEF2C, NF-κB, and PPARG, promoting processes such as adipocyte differentiation and brown fat accumulation (By similarity).
• CARM1 methylates the coactivator p300 at multiple arginine residues, modulating interactions with CREB and NCOA2/GRIP1, and inhibiting CREB-dependent transcription (PubMed:15731352). Related references: PMID:15731352
• It plays a role in pre-mRNA alternative splicing by methylating splicing factors (By similarity).
• Independently of its methyltransferase activity, CARM1 promotes replication fork progression by recruiting PARP1 to replication forks, leading to chromatin poly-ADP-ribosylation and reduced fork speed (PubMed:33412112). Related references: PMID:33412112
• CARM1 is localized in the nucleus and cytoplasm, and associates with chromosomes.
• It is overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin conjugation, detected through proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 486-603) targets the C-terminus of human PRMT4; verify sequence conservation in mouse and rat orthologs if cross-reactivity is essential.
• For Western blotting, expect a band near 66 kDa. Use KO-validated cell lysates to confirm antibody specificity.
• The antibody is suitable for immunoprecipitation and ELISA; optimize concentrations for your specific experimental conditions.
• PRMT4 undergoes various modifications; consider appropriate sample handling to preserve acetylation/phosphorylation states if studying these.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a comprehensive supply chain for IVD raw materials, including validated monoclonal antibodies such as this anti-PRMT4/CARM1 for epigenetic and transcription studies. Our portfolio spans matched antibody pairs, bulk ancillary reagents, and rare-target sourcing, all backed by technical and logistics support to accelerate your development from concept to clinic.