KO Validated Anti-PNP Rabbit Polyclonal Antibody for WB, ELISA - P00491

Core Product Specifications and Parameters

Biological Background: PNP (Purine Nucleoside Phosphorylase) Function and Localization

• PNP catalyzes the phosphorolytic breakdown of the N-glycosidic bond in beta-(deoxy)ribonucleoside molecules, forming free purine bases and pentose-1-phosphate, a key step in purine salvage. Related references: PMID:23438750, PMID:3029074, PMID:9305964
• Preferentially acts on 6-oxopurine nucleosides including inosine and guanosine, underscoring its substrate specificity. Related references: PMID:9305964
• The enzyme localizes predominantly to the cytoplasm and cytosol, consistent with its role in nucleoside metabolism.
• PNP is highly expressed in red blood cells and is overexpressed in the cytoplasm of erythrocytes from patients with hereditary non-spherocytic hemolytic anemia.
• The protein undergoes post-translational modifications including acetylation and phosphorylation, which may regulate its activity or stability.
• PNP is part of the purine salvage pathway; mutations in the PNP gene are associated with immunodeficiency disorders.

Experimental Guidance and Technical Tips

• The immunogen covers full-length human PNP (amino acids 1–289), indicating that the polyclonal antibody may detect both native and denatured forms of the protein.
• For WB analysis, consider using a positive control such as HeLa or HEK293 cell lysates, which express PNP, and validate band specificity at ~32 kDa.
• Given the cross-reactivity with mouse, the antibody can be considered for studies using mouse samples; however, validation in the specific tissue or cell line is recommended.
• For ELISA, use the antibody in a standard sandwich or indirect format and include appropriate negative controls to confirm target recognition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, research laboratories, and institutes with a comprehensive portfolio of IVD raw materials, including validated anti-PNP antibodies for nucleoside metabolism and immunology research. Our sourcing capabilities extend to optimized antibody pairs, bulk ancillary reagents, and rare target procurement, streamlining your development from discovery to clinical application. Contact us to discuss custom polyclonal or monoclonal antibody sourcing tailored to your assay requirements.