[KO Validated] Anti-PML Rabbit Monoclonal Antibody for WB, IP, IF/ICC, IHC-P, ELISA - P29590

Core Product Specifications and Parameters

Biological Background: PML Function and Localization

• Protein PML, also known as TRIM19, is a member of the tripartite motif (TRIM) family and functions as an E3 SUMO-protein ligase, playing a central role in the assembly of PML nuclear bodies (PML-NBs) and mediating sumoylation of target proteins.
• PML inhibits EIF4E-mediated mRNA nuclear export by reducing EIF4E affinity for the 5' m7G cap, contributing to its tumor suppressor function. Related references: PMID:11500381 PMID:11575918 PMID:18391071
• Alternative splicing generates multiple isoforms (e.g., PML-1 to PML-14), which exhibit distinct subcellular localizations and functional specializations; nuclear isoforms are involved in chromatin remodeling and gene regulation, while cytoplasmic isoforms mediate TGF-beta signaling and restrict viral replication.
• PML is predominantly localized in the nucleus where it forms PML-NBs, but can also be found in the nucleoplasm, cytoplasm, endoplasmic reticulum membrane, and early endosome membrane depending on isoform and cell context.
• Post-translational modifications such as SUMOylation, acetylation, and phosphorylation are critical for PML-NB dynamics and PML's functional interactions, influencing its role in apoptosis, circadian rhythm, and antiviral responses.
• PML acts as an E3 SUMO-protein ligase, sumoylating viral and cellular substrates to regulate antiviral innate immunity. Related references: PMID:20972456 PMID:28250117
• PML exhibits broad antiviral activity against both DNA and RNA viruses, including herpes simplex virus, influenza A, rabies, and hepatitis C, often by sequestering viral proteins within PML-NBs and inhibiting viral replication.
• As a tumor suppressor, PML regulates key signaling pathways by modulating the activity of p53, RB1, AKT, PTEN, and mTOR, contributing to growth suppression and senescence.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-185 of human PML, covering the N-terminal region which includes the RING finger and B-box domains; consider this when designing experiments to detect specific isoforms.
• For Western blot, a band at ~98 kDa is expected; however, due to multiple isoforms and sumoylation, additional bands may be observed. Validate with appropriate controls, especially when using knockout cell lines.
• In immunofluorescence, PML typically shows a punctate nuclear staining pattern characteristic of PML nuclear bodies; co-localization with markers like SP100 can confirm specificity.
• For immunohistochemistry, validate in human tissue samples and optimize antigen retrieval conditions to ensure robust nuclear staining. Note that PML expression can be low in some normal tissues.
• This antibody has been validated for knockout studies; include appropriate knockout controls to confirm specificity in your sample system.
• When using for immunoprecipitation or ELISA, consider the antibody’s reactivity with denatured vs. native PML and potential SUMO-dependent conformational epitopes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with high-quality IVD raw materials, including validated anti-PML antibodies for applications in oncology and infectious disease research. In addition to this rabbit monoclonal antibody, we offer a comprehensive portfolio of validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents to support your assay development. Our technical team can assist in sourcing rare-target reagents to accelerate your project from concept to clinical application.