[KO Validated] Anti-PKA RIIα (PRKAR2A) Polyclonal Antibody - P13861

Core Product Specifications and Parameters

Biological Background: PKA RIIα (PRKAR2A) Function and Localization

• PRKAR2A encodes the type II-alpha regulatory subunit of cAMP-dependent protein kinase (PKA), one of four regulatory isoforms that modulate PKA activity.
• Upon binding cAMP, the regulatory subunit dissociates from the catalytic subunits, releasing them to phosphorylate downstream targets in the cAMP signaling cascade.
• Type II regulatory chains tether PKA to specific subcellular sites by interacting with A-kinase anchoring proteins (AKAPs), such as MAP2 kinase, facilitating localized signaling.
• Expression of PRKAR2A varies across tissues; the four regulatory subunit types exhibit distinct tissue distributions and can be regulated constitutively or inducibly.
• The protein localizes to the cytoplasm and cell membrane, consistent with its function in compartmentalized cAMP signaling.
• The PRKAR2A gene undergoes alternative splicing and the protein is subject to acetylation and phosphorylation, with a calculated molecular weight of approximately 45.5 kDa (UniProt).

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning amino acids 1-404 of human PRKAR2A, designed to raise antibodies against multiple epitopes; this may enhance sensitivity across applications and species.
• For Western blotting, a starting dilution range of 1:500–1:2000 is often suitable; confirm specificity using knockout (KO) lysates, as this antibody has been KO validated.
• Given reactivity with mouse and rat, the antibody can be considered for cross-species studies; however, validate expression levels in your model system.
• In immunofluorescence/immunocytochemistry (IF/ICC), optimize fixation and permeabilization protocols, as the protein localizes to both cytoplasm and membrane.
• For ELISA, the antibody can be used as a detection reagent; pairing with a suitable capture antibody is recommended, and standard assay optimization is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated supplier of IVD raw materials, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For projects targeting PKA RIIα (PRKAR2A) in cAMP signaling research, CamelBio can assist with rare target sourcing and custom reagent development. From concept to clinic, CamelBio supports diagnostic manufacturers and research institutes with comprehensive technical services and consulting.