KO Validated Anti-PHD2/EGLN1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9GZT9

Core Product Specifications and Parameters

Biological Background: PHD2/EGLN1 Function and Localization

• Egl nine homolog 1 (EGLN1), commonly known as PHD2, is a hypoxia-inducible factor (HIF) prolyl hydroxylase and the primary oxygen sensor under normoxic conditions.
• PHD2 catalyzes the hydroxylation of specific proline residues within the oxygen-dependent degradation domains of HIF1A and HIF2A, leading to VHL-mediated ubiquitination and proteasomal degradation.
• Under hypoxia, PHD2 activity is reduced, allowing HIFα stabilization, nuclear translocation, and transcriptional activation of genes involved in angiogenesis, erythropoiesis, and metabolic adaptation.
• The enzyme belongs to the EGLN family of dioxygenases and contains a zinc-finger motif; it requires iron, 2-oxoglutarate, and ascorbate as cofactors for its catalytic function.
• Multiple isoforms are produced by alternative splicing, and post-translational modifications such as acetylation, phosphorylation, and S-nitrosylation have been reported.
• Subcellularly, PHD2 localizes predominantly to the cytoplasm but can also be detected in the nucleus.
• PHD2 is widely expressed, with highest levels in skeletal muscle, heart, brain, and kidney; it is also found in cardiac myocytes, aortic endothelial cells, and coronary artery smooth muscle.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (aa 1-100) of human PHD2; consider using a blocking peptide for specificity controls.
• For Western blotting, a starting dilution range of 1:500–1:2000 is typical; validate the optimal dilution in your sample system and note an expected band at ~46 kDa.
• For IHC-P, antigen retrieval may be required; test citrate or EDTA-based protocols and include appropriate positive controls (e.g., heart or skeletal muscle tissue).
• For IF/ICC, fixation and permeabilization conditions should be optimized; PHD2 is detected in the cytoplasm and nucleus.
• The antibody has been validated in KO samples; always include knockout or knockdown controls when investigating target specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined sourcing experience for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This PHD2/EGLN1 antibody supports research into hypoxia signaling and related disease areas, and our team can assist with procurement of complementary reagents to accelerate your assay development and diagnostic manufacturing.