[KO Validated] Anti-PEPCK/PCK2 Rabbit Polyclonal Antibody - Q16822

Core Product Specifications and Parameters

Biological Background: PEPCK/PCK2 Function and Localization

• PEPCK/PCK2 (Phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also known as PCK2, PEPCK2, and serine/threonine-protein kinase PCK2.
• Catalyzes the conversion of oxaloacetate to phosphoenolpyruvate, the rate-limiting step in gluconeogenesis and glyceroneogenesis. Related references: PMID:28955899
• Also functions as a serine/threonine-protein kinase: phosphorylates and activates ACSL4, thereby promoting ferroptosis. Related references: PMID:38720107
• Localized exclusively to the mitochondrion.
• Widely expressed across human tissues.
• Post‑translational modifications include acetylation and phosphorylation; alternative splicing generates multiple isoforms.
• Keywords highlight GTP‑binding, manganese‑dependent lyase activity, and disease variant associations.

Experimental Guidance and Technical Tips

• The immunogen spans the N‑terminal 280 amino acids; expect detection of full‑length PCK2 (~71 kDa) and potentially some truncated forms in WB.
• In IHC‑P and IF/ICC, a mitochondrial staining pattern should be anticipated; validate in the relevant sample system.
• Polyclonal nature may lead to lot‑to‑lot variation; confirm specificity with appropriate controls (e.g., knockout lysates).
• Cross‑reactivity with mouse and rat makes this antibody suitable for orthologous studies; perform a pilot experiment to confirm reactivity in your experimental setup.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to validated IVD raw materials. The PEPCK/PCK2 antibody supports research in glucose metabolism, ferroptosis, and related disease areas. As a B2B sourcing partner, CamelBio can supply this polyclonal antibody along with optimized antibody pairs, bulk ancillary reagents, and custom procurement for rare‑target raw materials—helping you accelerate your project from concept to clinical application.