[KO Validated] Anti-PDLIM5 Rabbit Monoclonal Antibody for WB, IP, IHC-P, ELISA - Q96HC4

Core Product Specifications and Parameters

Biological Background: PDZ and LIM domain protein 5 Function and Localization

• PDLIM5 (also known as ENH, L9, LIM) is a PDZ and LIM domain-containing protein expressed as multiple isoforms through alternative splicing, with isoform 1 containing an N-terminal PDZ domain and three C-terminal LIM domains.
• As a cardiac-specific scaffolding protein, PDLIM5 tethers protein kinase C to the Z-disk region, playing a critical role in heart development and regulating cardiomyocyte expansion.
• In neurons, isoform 1 contributes to dendritic spine morphogenesis by restraining postsynaptic growth of excitatory synapses, promoting spine thinning and elongation.
• Subcellularly, PDLIM5 localizes to the postsynaptic density, presynapse, cytoplasm, and cytosol, consistent with its roles at cell junctions and signaling complexes.
• Expression is predominantly in heart and skeletal muscle, but notably increased in the brain of patients with bipolar disorder, schizophrenia, and major depression, implicating it in neuropsychiatric disorders.
• The protein is subject to post-translational modifications including phosphorylation and acetylation, and contains metal-binding LIM domains, which are key to its structural and functional versatility.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 100-300 of human PDLIM5 (NP_006448.4), a region that includes part of the PDZ domain and the first LIM domain, suggesting potential reactivity with multiple isoforms; validate in your specific sample system.
• For Western Blot, note that the predicted molecular weight is ~64 kDa, but multiple isoforms and post-translational modifications result in an observed range of 15-68 kDa; use appropriate loading controls and molecular weight markers.
• In Immunohistochemistry (IHC-P), positive staining is expected in heart and skeletal muscle, as well as brain tissue; consider antigen retrieval conditions and validate with known positive controls.
• For Immunoprecipitation, ensure the antibody is compatible with your lysis buffer and that the target protein is expressed at sufficient levels in your sample.
• ELISA performance may depend on the coating antigen and detection system; consider titrating the antibody for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a global IVD raw-material partner, providing diagnostic manufacturers, laboratories, and research institutes with one-stop access to validated antibodies, bulk ancillary reagents, and rare target sourcing. This anti-PDLIM5 antibody supports cardiovascular and neuroscience research applications. For projects requiring customized or large-scale production, CamelBio offers expert consulting and supply chain solutions from concept to clinic.