[KO Validated] Anti-PAK2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q13177

Core Product Specifications and Parameters

Biological Background: PAK2 Function and Localization

• PAK2 is a serine/threonine-protein kinase involved in cytoskeleton regulation, cell motility, cell cycle progression, apoptosis, and proliferation. Related references: PMID:12853446, PMID:16617111, PMID:19273597
• It acts as a downstream effector of the small GTPases CDC42 and RAC1; binding of active GTPases triggers conformational change and autophosphorylation. Related references: PMID:7744004
• Full-length PAK2 promotes cell survival and growth, whereas caspase-mediated cleavage yields the pro-apoptotic p34 fragment that translocates to the nucleus and activates the JNK pathway. Related references: PMID:7744004, PMID:12853446, PMID:16617111, PMID:9171063
• PAK2 phosphorylates diverse substrates including MAPK4/6, JUN, histone H4 (facilitating nucleosome assembly), BAD, ribosomal protein S6, and CASP7. Related references: PMID:21317288, PMID:21177766, PMID:21724829, PMID:21555521, PMID:27889207
• Beyond its catalytic activity, PAK2 binds ARHGEF7 and GIT1 to regulate spindle orientation during mitosis. Related references: PMID:19273597, PMID:19923322
• The protein localizes to the cytoplasm, nucleus, perinuclear region, and membranes.
• Ubiquitously expressed, with higher levels observed in skeletal muscle, ovary, thymus, and spleen.
• PAK2 undergoes acetylation, myristoylation, and ubiquitination; it is an allosteric enzyme with ATP-binding activity and is also known as p58, PAK65, gamma-PAK, and PAK-2.

Experimental Guidance and Technical Tips

• For Western blotting (WB), the predicted molecular weight of full-length PAK2 is ~58 kDa; under apoptotic conditions, the cleaved ~34 kDa fragment may be observed. Ensure appropriate controls are included.
• In immunofluorescence/immunocytochemistry (IF/ICC), PAK2 exhibits cytoplasmic and nuclear staining, with possible enrichment at perinuclear regions and membranes.
• For ELISA, the immunogen corresponds to the N-terminal region (aa 1–212); combining with antibodies against other epitopes may enable sandwich ELISA development.
• As a polyclonal antibody, lot-to-lot consistency should be verified; consider using PAK2 knockout cell lines as negative controls to confirm specificity in your assays.
• Standard blocking and dilution optimization (e.g., starting at 1:500 for WB) is recommended; empirical testing in the relevant sample system is essential.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research laboratories, offering a wide range of IVD raw materials from validated antibody pairs to bulk ancillary reagents. This anti-PAK2 polyclonal antibody supports studies in cell signaling, cytoskeleton dynamics, and apoptosis research. Our team can also assist with custom sourcing for rare-target antibodies, recombinant proteins, and assay development tools, bridging the gap from concept to clinical application.