[KO Validated] Anti-Nucleobindin-1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q02818

Core Product Specifications and Parameters

Biological Background: Nucleobindin-1 Function and Localization

• Nucleobindin-1 (NUCB1), also known as CALNUC, is a major calcium-binding protein predominantly localized to the Golgi apparatus, where it may regulate calcium homeostasis (By similarity).
• It acts as a non-receptor guanine nucleotide exchange factor (GEF), binding to and activating alpha subunits of G proteins, thus linking calcium signaling to G protein-mediated pathways (By similarity).
• Subcellular localization encompasses the cis-Golgi network membrane, cytoplasm, and secretion, indicating dynamic roles in intracellular trafficking and extracellular signaling.
• NUCB1 contains EF-hand calcium-binding domains, a coiled-coil region, and undergoes glycosylation and phosphorylation, which modulate its function and stability.
• Tissue expression is broad, including fetal and adult heart, lung, liver, kidney, brain, and adult skeletal muscle, placenta, and pancreas, underscoring its ubiquitous biological importance.
• Additional in vitro DNA-binding activity and structural characterization (3D-structure available) highlight its multifunctional nature.

Experimental Guidance and Technical Tips

• The immunogen corresponds to aa 302–461 of human NUCB1; consider using a recombinant fragment or full-length protein as a positive control in assays.
• For Western blot, a predominant band at ~54 kDa is expected; minor bands from post-translational modifications or isoforms may be observed, and antibody specificity can be confirmed with KO cell lysates.
• In immunofluorescence/ICC, gentle permeabilization and Golgi-specific counterstains (e.g., GM130) help confirm localization; avoid harsh detergents that disrupt Golgi membranes.
• For ELISA applications, titrate the antibody and use appropriate blocking to minimize background; pairing with a capture antibody may enhance sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, technical services, and consulting, supporting diagnostic developers from concept to clinic. For calcium signaling and Golgi biology targets like nucleobindin-1, our portfolio includes validated recombinant antibodies, matched antibody pairs, and bulk ancillary reagents. With expertise in rare target sourcing, CamelBio serves as a reliable bridge for your raw-material needs.