[KO Validated] Anti-NRD1 Rabbit Polyclonal Antibody for WB and ELISA - O43847

Core Product Specifications and Parameters

Biological Background: Nardilysin Function and Localization

• Nardilysin, also known as N-arginine dibasic convertase (NRD convertase, NRD-C), is encoded by the NRDC (NRD1) gene in humans.
• It functions as a zinc-dependent metalloprotease that cleaves peptide substrates on the N-terminus of arginine residues in dibasic pairs.
• NRD1 is a critical activator of BACE1- and ADAM17-mediated pro-neuregulin ectodomain shedding, promoting axonal maturation and myelination.
• It is required for the proper functioning of 2-oxoglutarate dehydrogenase (OGDH), linking it to mitochondrial energy metabolism.
• Subcellularly, nardilysin localizes to mitochondria and cell projections such as dendrites.
• Expression is primarily observed in adult heart, skeletal muscle, and testis, with lower levels in other tissues.
• The protein undergoes alternative splicing and phosphorylation, and contains a signal peptide.

Experimental Guidance and Technical Tips

• The antibody is knockout-validated; consider using KO cell or tissue lysates as negative controls to confirm specificity in WB.
• Immunogen region (aa 160–400) is centrally located; for competitive assays, peptides spanning this region may be useful.
• For WB, NRD1 has a predicted molecular weight of ~132 kDa; use appropriate gel gradients and transfer conditions for high-molecular-weight proteins.
• Recommended positive controls include heart, skeletal muscle, or testis lysates due to high target expression.
• Cross-reactivity is reported for human, mouse, and rat; for other species, align the immunogen sequence beforehand and validate empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials, including validated antibodies for targets like nardilysin. Our portfolio covers polyclonal and monoclonal antibodies, optimized for applications such as WB and ELISA, along with bulk ancillary reagents. For rare or emerging targets, we offer tailored sourcing solutions to accelerate your assay development from concept to clinic.