[KO Validated] Anti-NRBF2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q96F24

Core Product Specifications and Parameters

Biological Background: NRBF2 Function and Localization

• NRBF2 (Nuclear receptor-binding factor 2), also known as Comodulator of PPAR and RXR (COPR), may modulate transcriptional activation by target nuclear receptors and can act as a transcriptional activator in vitro.
• It is implicated in the induction of starvation-induced autophagy, associating with the PI3K complex I (PI3KC3-C1).
  Related references:
  PMID:24785657
• Proposed to negatively regulate basal and starvation-induced autophagy and to inhibit PIK3C3 activity by modulating interactions in PI3KC3-C1.
  Related references:
  PMID:25086043
• May be involved in autophagosome biogenesis.
• Subcellular localization includes the nucleus, cytoplasm, and autophagosomes.
• Detected in keratinocytes, liver, placenta, and a subset of cells in pediatric medulloblastoma.
• The protein undergoes alternative splicing and phosphorylation, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–287, covering nearly the full-length protein; this design likely enables recognition of multiple isoforms and detection of endogenous NRBF2 across applications.
• For Western blotting, a band around 32 kDa is expected; however, additional bands may appear due to post-translational modifications or splice variants. Validate with knockout controls where available.
• In IHC-P and IF/ICC, consider testing on tissues or cell lines known to express NRBF2 (e.g., liver, placenta, keratinocytes) and optimize antigen retrieval for paraffin sections.
• For ELISA, pairing with a suitable detection antibody may enable quantification; validate specificity with recombinant protein or known positive samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio that includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. This anti-NRBF2 antibody supports research in autophagy and transcriptional regulation, and CamelBio can facilitate the procurement of matched reagents and related raw materials to accelerate diagnostic development from concept to clinic.