[KO Validated] Anti-NOSIP Rabbit Polyclonal Antibody for WB, ELISA - Q9Y314

Core Product Specifications and Parameters

Biological Background: NOSIP Function and Localization

• NOSIP (Nitric oxide synthase-interacting protein), also referred to as CGI-25, eNOS-interacting protein, or RING-type E3 ubiquitin transferase NOSIP, is encoded by the NOSIP gene.
• Functions as an E3 ubiquitin-protein ligase, catalyzing monoubiquitination of serine/threonine-protein phosphatase 2A (PP2A) catalytic subunits PPP2CA/PPP2CB (By similarity).
• Negatively regulates nitric oxide production by inducing translocation of NOS1 and NOS3 to the actin cytoskeleton and inhibiting their enzymatic activity. Related references: PMID:11149895 PMID:15548660 PMID:16135813
• Essential for proper development of the forebrain, the eye, and the face (By similarity).
• Predominantly localized in the cytoplasm and nucleus.
• Expressed in heart, brain, and lung, and present in endothelial cells at the protein level.
• Phosphoprotein and developmental protein, with additional roles in the ubiquitin conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen spans nearly full-length NOSIP (aa 1-301), suggesting potential to recognize both native and denatured forms; validate in your specific assay conditions.
• For WB, consider using a loading control validated in the same species and tissue, and verify that the observed band (~33 kDa) matches the expected molecular weight.
• For ELISA, optimize coating conditions and detection system for your specific assay format.
• Cross-reactivity with species other than human has not been confirmed; if using non-human samples, validate reactivity independently.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, monoclonal/polyclonal antibodies, bulk reagents, and rare-target sourcing to support research in nitric oxide signaling, developmental biology, and ubiquitination pathways. Whether you need this anti-NOSIP antibody for assay development or complementary reagents, CamelBio streamlines your procurement from concept to clinic.