[KO Validated] Anti-NDUFC2 Polyclonal Antibody for WB and ELISA - O95298

Core Product Specifications and Parameters

Biological Background: NDUFC2 Function and Localization

• NDUFC2 encodes NADH dehydrogenase [ubiquinone] 1 subunit C2, also designated CI-B14.5b, HLC-1, and B14.5b; it is a nuclear-encoded accessory subunit of mitochondrial complex I.
• The protein functions as an assembly factor for complex I, being essential for the proper formation and stability of the enzyme complex but not directly involved in catalysis.
• Complex I accepts electrons from NADH and transfers them to ubiquinone, a critical step in the mitochondrial respiratory chain that drives oxidative phosphorylation.
• NDUFC2 localizes to the mitochondrial inner membrane, consistent with its role in electron transport and energy metabolism.
• It contains a single transmembrane helix, anchoring it within the membrane, and belongs to the complex I subunit family.
• Alternative splicing yields multiple transcript variants, and mutations are linked to primary mitochondrial disease.
• Associated keywords include electron transport, respiratory chain, mitochondrion inner membrane, and disease variant.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning the N-terminal region (residues 1–100); consider using a blocking peptide or knockout validation to confirm specificity in your experimental system.
• For Western blot, mitochondrial-enriched fractions or total cell lysates from HeLa, HEK293, or liver tissue may enhance detection of the ~14 kDa band.
• In ELISA, perform a checkerboard titration to optimize coating antigen concentration and primary antibody dilution; use a BSA or casein-based block.
• Cross-reactivity with mouse and rat is reported, but confirm reactivity in your specific model by including appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and research labs with comprehensive access to IVD raw materials. This NDUFC2 antibody is suitable for investigating mitochondrial complex I function and respiratory chain disorders. Beyond this product, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, and can source rare-target raw materials to accelerate your assay development.