KO Validated Anti-N-terminal alpha-synuclein Monoclonal Antibody [MJFF-B-3A4] for WB, IF/ICC, ELISA - P37840

Core Product Specifications and Parameters

Biological Background: Alpha-synuclein Function and Localization

• Alpha-synuclein (SNCA), also known as NACP or PARK1, is a neuronal protein highly expressed in presynaptic terminals in the central nervous system.
• It plays a critical role in synaptic vesicle trafficking and neurotransmitter release by enhancing vesicle priming, fusion, and dilation of exocytotic fusion pores. Related references: PMID:28288128, PMID:30404828
• Mechanistically, it increases local Ca2+ release from microdomains to enhance ATP-induced exocytosis. Related references: PMID:30404828
• In its multimeric membrane-bound state, alpha-synuclein acts as a molecular chaperone that assists in the folding of SNARE proteins, crucial for sustaining SNARE-complex assembly during aging. Related references: PMID:20798282
• It also regulates dopamine neurotransmission by interacting with the dopamine transporter (DAT1) and modulating its activity. Related references: PMID:26442590
• Subcellularly, alpha-synuclein localizes to the cytoplasm, membrane, nucleus, synapse, and is secreted; it is also found in cell projections like axons.
• Mutations in SNCA are linked to Parkinson disease and other synucleinopathies, with alpha-synuclein accumulating in Lewy bodies.

Experimental Guidance and Technical Tips

• For Western blot, titrate the antibody to determine optimal dilution for your sample type; the expected band is approximately 14 kDa.
• For immunofluorescence, optimize fixation and permeabilization conditions suitable for synaptic protein detection.
• For ELISA, consider pairing this antibody with a detection antibody to develop a quantitative assay.
• The KO validation underscores specificity; use appropriate knockout controls to confirm target engagement.
• With confirmed cross-reactivity in mouse and rat, this antibody is suitable for studies in rodent models of neurodegeneration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Whether you are developing assays for neurodegeneration biomarkers like alpha-synuclein or need access to rare target materials, our one-stop platform streamlines your procurement from concept to clinic.