[KO Validated] Anti-N-Cadherin Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P19022

Core Product Specifications and Parameters

Biological Background: Cadherin-2 Function and Localization

• Cadherin-2 (CDH2), also known as N-cadherin or CDw325, is a calcium-dependent cell adhesion protein that mediates homotypic cell-cell adhesion through dimerization with CDH2 chains from adjacent cells, contributing to the sorting of heterogeneous cell types.
• As a regulator of neural stem cell quiescence, CDH2 anchors neural stem cells to ependymocytes in the adult subependymal zone; cleavage by MMP24 disrupts this anchorage, modulating stem cell quiescence.
• CDH2 plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem cells, promoting process formation by neural crest stem cells.
• The protein is required for proper neurite branching and pre- and postsynaptic organization, and in hippocampal neurons, it may regulate dendritic spine density.
• Subcellularly, CDH2 localizes to the cell membrane, sarcolemma, cell junctions (adherens junctions, desmosomes), cell surface, and cell projections such as axons.
• Key post-translational modifications and features include alternative splicing, a signal peptide, transmembrane helix, repeat domains, cleavage on pair of basic residues, glycoprotein status, and phosphorylation.
• CDH2 is implicated in various cellular processes and diseases, with keywords linking it to calcium binding, cardiomyopathy, intellectual disability, and cell adhesion.

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody was raised against a synthetic peptide corresponding to the extracellular region of human N-Cadherin (amino acids 100-200); consider confirming specificity with a blocking peptide or competition assay.
• For Western blot, N-Cadherin has a predicted molecular weight of approximately 100 kDa; optimize gel percentages and transfer conditions, and include appropriate molecular weight markers and loading controls.
• The antibody is validated for knockout (KO) applications, which enhances specificity assurance; it is advisable to include KO cell lysates as negative controls in your experiments.
• When performing immunofluorescence (IF-P) or immunohistochemistry (IHC-P), optimize fixation/permeabilization and antigen retrieval (for IHC-P) protocols for your target tissues.
• The reported cross-reactivity with mouse and rat supports use in rodent models, but validation in the specific tissue or cell line of interest is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes one-stop access to IVD raw materials, including this high-quality N-Cadherin monoclonal antibody for cell adhesion and neuroscience applications. We provide validated antibody pairs, bulk ancillary reagents, and customized sourcing solutions to streamline your development workflow. Partner with CamelBio to bridge the gap from target discovery to clinical readiness with reliable, scalable raw material supply.