[KO Validated] Anti-MTH1 Rabbit Polyclonal Antibody for WB, IP, ELISA - P36639

Core Product Specifications and Parameters

Biological Background: MTH1 Function and Localization

• MTH1 (NUDT1) is an oxidized purine nucleoside triphosphate hydrolase, also known as 8-oxo-dGTPase, that sanitizes the nucleotide pool to prevent DNA damage. Related references: PMID:10608900, PMID:12857738, PMID:22556419
• It catalyzes the hydrolysis of 2-oxo-dATP into 2-oxo-dAMP and exhibits strong hydrolase activity toward 2-oxo-ATP, 8-oxo-dGTP, and 8-oxo-dATP. Related references: PMID:10373420, PMID:11139615
• By removing oxidized purine nucleoside triphosphates, MTH1 prevents their incorporation into DNA and suppresses A:T to C:G and G:C to T:A transversion mutations. Related references: PMID:10373420, PMID:10608900, PMID:11756418
• MTH1 also hydrolyzes methylated purine nucleoside triphosphates (e.g., O6-methyl-dGTP), blocking their integration into nucleic acids. Related references: PMID:30304478, PMID:32144205
• Through these antimutagenic activities, MTH1 protects cells from oxidative stress-induced DNA damage. Related references: PMID:10608900, PMID:12857738, PMID:24695224
• MTH1 localizes to the cytoplasm, cytosol, mitochondrial matrix, and nucleus, reflecting its role in sanitizing nucleotides in multiple compartments.
• It is widely expressed, with highest levels in thymus, testis, embryo, and proliferating blood lymphocytes.
• MTH1 is a magnesium-dependent hydrolase belonging to the Nudix family; it contains an alternative initiation site, a transit peptide for mitochondrial targeting, and has reported RNA-binding activity.

Experimental Guidance and Technical Tips

• For Western blot, consider using whole cell lysates from human cell lines expressing endogenous MTH1; validate detection of the ~18 kDa band.
• For immunoprecipitation, titrate the antibody amount and use appropriate negative controls.
• Knockout validation confirms specificity; include a KO cell line as a negative control to ensure band identity.
• For ELISA applications, test the antibody as a capture or detection reagent in a matched pair format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For MTH1 research and oxidative stress-related diagnostic development, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Partner with CamelBio to accelerate your IVD assay development from discovery to commercialization.