KO Validated Anti-MTA2 Rabbit Polyclonal Antibody for WB, ELISA, ChIP - O94776

Core Product Specifications and Parameters

Biological Background: MTA2 Function and Localization

• MTA2, also known as MTA1-L1 or PID, is a metastasis-associated protein encoded by the MTA2 gene (GeneID: 9219).
• It functions as a transcriptional coregulator within the NuRD (nucleosome remodeling and deacetylase) complex, which remodels chromatin and represses transcription. Related references: PMID:16428440, PMID:28977666
• The protein localizes to the nucleus, consistent with its role in chromatin modification.
• MTA2 contains zinc-finger domains that mediate DNA-binding and metal ion interactions.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination (isopeptide bonds), which may regulate its activity and stability.
• It is widely expressed in human tissues, indicating broad biological significance.
• The predicted molecular weight is 75 kDa, consistent with observed protein weight.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fragment (aa 60-180) of human MTA2; consider verifying antibody specificity using a blocking peptide or knockout cell line if available.
• For Western blot, a band around 75 kDa is expected; optimize primary antibody dilution and run appropriate positive controls (e.g., HeLa, MCF-7 cell lysates) as MTA2 is widely expressed.
• In ELISA, titrate the antibody to determine optimal working concentration for your specific assay format.
• For ChIP, validate the antibody's ability to enrich MTA2-bound chromatin under your experimental conditions, including appropriate positive and negative control regions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our one-stop sourcing platform supports diagnostic manufacturers and research labs with high-quality antibodies like the Anti-MTA2 polyclonal antibody, facilitating chromatin remodeling and cancer research. Explore seamless procurement and technical support for your development needs.