KO Validated Anti-MLH1 Monoclonal Antibody for WB, IHC-P, ELISA - P40692

Core Product Specifications and Parameters

Biological Background: MLH1 Function and Localization

• DNA mismatch repair protein Mlh1 (MutL protein homolog 1) is the catalytic subunit of the post-replicative DNA mismatch repair (MMR) system, forming the MutL alpha heterodimer with PMS2 and the MutL gamma heterodimer with MLH3.
• MutL alpha is recruited to mismatched DNA by the MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) complexes; it then activates PMS2 endonuclease to introduce strand breaks, initiating mismatch removal and repair.
• MLH1 localizes predominantly to the nucleus and is associated with chromosomes during DNA replication and repair processes.
• Expressed in multiple tissues including colon, lymphocytes, breast, lung, spleen, testis, prostate, thyroid, gall bladder, and heart.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin conjugation, which may regulate its stability and function.
• MLH1 is classified as a tumor suppressor; germline mutations are linked to hereditary nonpolyposis colorectal cancer (HNPCC), also known as Lynch syndrome.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 350–450 of human MLH1; epitope specificity should be verified for intended applications.
• For Western blot, the antibody detects a band at approximately 85 kDa; validate using human or mouse cell lysates with known MLH1 expression and include knockout controls if available.
• In immunohistochemistry (IHC-P), optimize antigen retrieval conditions and titration; compare staining patterns with known tissue distribution and use knockout tissue sections as negative controls.
• For ELISA, pair this antibody with a suitable detection reagent and validate using recombinant MLH1 protein to establish sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a single, integrated source for validated IVD raw materials, including high-quality monoclonal antibodies like this anti-MLH1 product for DNA mismatch repair and cancer research. Our comprehensive portfolio encompasses optimized antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets—streamlining your assay development from concept to clinic. Partner with us to accelerate your MLH1-based projects with reliable raw materials and expert technical support.