KO Validated anti-macroH2A.1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O75367

Core Product Specifications and Parameters

Biological Background: macroH2A.1 Function and Localization

• Core histone macro-H2A.1 is a variant histone H2A that replaces conventional H2A in a subset of nucleosomes, thereby repressing transcription. It compacts DNA into chromatin, limiting DNA accessibility for transcription, DNA repair, DNA replication, and chromosomal stability. Related references: PMID:12718888, PMID:15621527, PMID:16428466, PMID:15897469
• Inhibits binding of transcription factors (e.g., NF-kappa-B) and interferes with SWI/SNF remodeling complexes. Also inhibits histone acetylation by EP300 and recruits class I HDACs, inducing a hypoacetylated chromatin state. Related references: PMID:12718888, PMID:16428466, PMID:16107708
• Isoform 1 specifically binds poly-ADP-ribose and O-acetyl-ADP-ribose, playing a key role in NAD+ metabolism by capping PARP1-generated poly-ADP-ribose chains, thus limiting nuclear NAD+ consumption and promoting mitochondrial respiration. Increases expression of redox metabolism genes like SOD3. Isoform 2 lacks this binding capability and represses SOD3 expression. Related references: PMID:15902274, PMID:23022728
• Involved in stable X chromosome inactivation. Related references: PMID:15897469
• Localized to the nucleus and associated with chromosomes.
• Widely expressed across tissues.
• Post-translationally modified by acetylation, methylation, phosphorylation, and ubiquitin-like conjugation (SUMOylation/ubiquitination). Contains isopeptide bonds.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 123-372 of human macroH2A.1 (NP_613258.2). This region includes the non-histone domain, which may confer isoform specificity. Validate the antibody’s recognition of relevant isoforms in your experimental system.
• This antibody is suitable for WB, IHC-P, IF/ICC, and ELISA. Consider optimizing antibody dilution and detection conditions for each application. For WB, a band around 39 kDa is expected.
• The antibody is KO validated; consider including appropriate controls (e.g., lysates from wild-type and KO cells) to confirm specificity in your assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. This anti-macroH2A.1 polyclonal antibody supports research into chromatin biology, epigenetics, and gene regulation. Our supply capabilities include validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare-target raw materials. Contact us to streamline your diagnostic development and research procurement.