[KO Validated] Anti-LITAF Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - Q99732

Core Product Specifications and Parameters

Biological Background: LITAF Function and Localization

• LITAF (Lipopolysaccharide-induced tumor necrosis factor-alpha factor), also known as PIG7 and SIMPLE (Small Integral Membrane Protein of Lysosome/Late Endosome), is a 161-amino acid protein encoded by the LITAF gene in humans.
• It plays a critical role in endosomal protein trafficking and lysosomal degradation, specifically in targeting membrane proteins such as EGFR and ERBB3 for degradation via the ESCRT pathway.
  Related references:
  PMID:23166352
• LITAF interacts with NEDD4, suggesting a role in ubiquitin-mediated protein degradation.
  Related references:
  PMID:15776429
• In the nucleus, LITAF may act as a transcription factor; it binds DNA and cooperates with STAT6 to regulate the expression of multiple cytokines, including TNF, CCL2, CCL5, CXCL1, IL1A, and IL10.
  Related references:
  PMID:10200294
  PMID:15793005
• The protein localizes to various membrane compartments including the lysosome, early and late endosomes, Golgi apparatus, and cell membrane; it is also found in the cytoplasm and nucleus.
• Tissue specificity: LITAF is ubiquitously expressed, with highest levels in the placenta, peripheral blood leukocytes, lymph nodes, and spleen.
• Mutations in LITAF are associated with Charcot-Marie-Tooth disease type 1C, a demyelinating peripheral neuropathy.
• LITAF is a phosphoprotein and contains zinc-binding motifs, indicative of its regulation by post-translational modifications and metal coordination.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human LITAF protein (amino acids 1-161), suggesting the antibody may recognize multiple epitopes and native conformations.
• For Western blotting, a band around 17 kDa is expected; consider using appropriate controls such as knockout lysates (this product is KO-validated) to confirm specificity.
• For immunohistochemistry (IHC-P), antigen retrieval conditions may need optimization; inclusion of positive and negative tissue controls is advised.
• The antibody cross-reacts with mouse LITAF, enabling studies in mouse models; validate in the relevant sample system before large-scale experiments.
• For immunoprecipitation (IP) and ELISA, titrate the antibody concentration to achieve optimal signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to providing diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials and technical support. This anti-LITAF polyclonal antibody supports investigations into endosomal trafficking, cytokine regulation, and neurodegenerative diseases such as Charcot-Marie-Tooth disease. In addition to this product, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets to streamline your entire assay development workflow from concept to clinic.