[KO Validated] Anti-LDHB Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P07195

Core Product Specifications and Parameters

Biological Background: LDHB Function and Localization

• L-lactate dehydrogenase B chain (LDHB), also known as LDH heart subunit (LDH-H), is an enzyme belonging to the lactate dehydrogenase family. It is also identified as renal carcinoma antigen NY-REN-46.
• LDHB catalyzes the interconversion of pyruvate and lactate with the simultaneous interconversion of NADH and NAD(+). This reaction is stereospecific and plays a key role in anaerobic glycolysis.
• In humans, LDHB is predominantly expressed in aerobic tissues, particularly cardiac muscle, reflecting its role in efficient lactate utilization under aerobic conditions.
• Subcellularly, LDHB localizes to the cytoplasm and the mitochondrial inner membrane, suggesting involvement in both cytoplasmic glycolysis and mitochondrial energy metabolism.
• Post-translational modifications such as acetylation and phosphorylation have been reported, which may regulate its enzymatic activity or subcellular distribution.
• Mutations in LDHB have been associated with disease variants, underscoring its clinical relevance in metabolic and muscle disorders.
• LDHB is a reference proteome member and has been identified through direct protein sequencing and proteomics identification, confirming its expression and sequence at the protein level.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein corresponding to amino acids 2–334 of human LDHB (full-length mature protein). This broad coverage may yield high sensitivity and strong signals in Western blot (WB). Consider optimizing primary and secondary antibody concentrations for your specific sample type.
• For immunofluorescence (IF-P), LDHB is expected to localize to the cytoplasm and mitochondria. Include co-staining with a mitochondrial marker (e.g., MitoTracker) and appropriate controls to confirm specificity. Permeabilization protocols may affect mitochondrial staining.
• In ELISA applications, the polyclonal nature of this antibody may be suitable for both capture and detection roles, depending on the assay format. Cross-species reactivity with mouse and rat supports translational or model organism studies. Validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers, clinical laboratories, and research institutes with reliable one-stop access to critical raw materials and technical support across the entire diagnostic development pathway. Our LDHB polyclonal antibody and related products—including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—support robust assay development in metabolism, cardiac disease, and oncology. Whether you need single antibodies or full raw-material panels, CamelBio bridges your projects from concept to clinic.