[KO Validated] Anti-LAMP2 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, FC, ELISA - P13473

Core Product Specifications and Parameters

Biological Background: LAMP2 Function and Localization

• LAMP2 (Lysosome-associated membrane glycoprotein 2) is a highly glycosylated type I transmembrane protein, also known as CD107b or LGP-96. Three main isoforms (LAMP-2A, -2B, and -2C) are produced by alternative splicing, each with distinct tissue distributions and roles in lysosomal function.
• It plays an essential role in lysosome biogenesis, lysosomal pH regulation, and autophagy. LAMP2 directly inhibits the proton channel TMEM175, facilitating lysosomal acidification required for optimal hydrolase activity. Related references: PMID:11082038, PMID:18644871, PMID:24880125, PMID:37390818
• LAMP2 is a central receptor in chaperone-mediated autophagy (CMA). It binds substrate proteins (e.g., GAPDH, GPX4, NLRP3) that have been recognized by the chaperone HSC70, and targets them for lysosomal degradation, especially under starvation. Related references: PMID:11082038, PMID:18644871, PMID:24880125, PMID:36586411
• During macroautophagy, LAMP2 is required for the fusion of autophagosomes with lysosomes; cells lacking LAMP2 fail to recruit STX17 to autophagosomes, preventing degradation of autophagic cargo. Related references: PMID:27628032
• LAMP2 contributes to adaptive immunity by facilitating MHC class II-mediated presentation of exogenous antigens; it promotes the generation of antigenic peptides within the endosomal/lysosomal compartment. Related references: PMID:15894275, PMID:20518820
• During mumps virus infection, LAMP2 supports the FURIN-mediated cleavage of the viral fusion protein F by interacting with both FURIN and the unprocessed viral protein.
• LAMP2 localizes primarily to the lysosome membrane and, to a lesser extent, to endosomes, autophagosomes, and the plasma membrane.
• Isoform‑specific tissue expression: LAMP-2A is abundant in placenta, lung, and liver; LAMP-2B is widely expressed in tissues including spleen, thymus, prostate, and testis; LAMP-2C is found in small intestine, colon, heart, brain, and skeletal muscle. Related references: PMID:26856698, PMID:7488019

Experimental Guidance and Technical Tips

• The immunogen corresponds to the luminal domain (amino acids 29–375) of human LAMP2, targeting an extracellular epitope accessible in non‑permeabilized samples for surface staining (e.g., FC) and in permeabilized samples for total detection.
• For Western blotting, the predicted core protein weight is 45 kDa, but extensive N‑ and O‑linked glycosylation typically results in a broad band migrating at ~90–110 kDa; use a suitable positive control such as HeLa or HepG2 whole‑cell lysate.
• In immunofluorescence and IHC, fixation/permeabilization conditions must be optimized to access the lysosomal membrane compartment; consider co‑staining with lysosomal markers (e.g., LAMP1) to confirm localization.
• For flow cytometry, intracellular staining (FC intra) requires cell permeabilization; compatibility with fluorescence‑conjugated secondary antibodies and fixation protocols should be validated in your specific cellular context.
• This antibody has been validated for knockout confirmation; always include appropriate knockout or knockdown controls to verify antibody specificity in your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one‑stop partner for IVD raw materials, offering a comprehensive portfolio of validated antibodies, bulk ancillary reagents, and rare‑target sourcing services. This Anti‑LAMP2 monoclonal antibody supports fundamental research in autophagy, lysosomal biology, and lysosomal storage disorders, and is available alongside customized assay development and production‑scale supply. Leverage our end‑to‑end expertise to accelerate your diagnostic development from concept to clinic.