[KO Validated] Anti-LAMP2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P13473

Core Product Specifications and Parameters

Biological Background: LAMP2 Function and Localization

• LAMP2 (Lysosome-associated membrane glycoprotein 2), also referred to as CD107b and LGP-96, is a heavily glycosylated lysosomal membrane protein. Three major isoforms (LAMP-2A, LAMP-2B, LAMP-2C) are produced by alternative splicing, each with distinct tissue-specific expression and regulatory roles.
• Core function: LAMP2 is essential for lysosome biogenesis, lysosomal pH regulation (via direct inhibition of TMEM175), and autophagy, particularly chaperone-mediated autophagy (CMA). It binds chaperone-targeted proteins (e.g., GAPDH, GPX4, NLRP3) and targets them for lysosomal degradation. (PubMed:11082038, PubMed:18644871, PubMed:24880125, PubMed:37390818, PubMed:36586411) Related references: PMID:11082038, PMID:18644871, PMID:24880125, PMID:37390818, PMID:36586411
• Facilitates autophagosome-lysosome fusion by promoting the accumulation of STX17 on autophagosomes, a process required for autophagic degradation. (PubMed:27628032) Related references: PMID:27628032
• Plays a critical role in MHC class II-mediated presentation of exogenous antigens, but not endogenous antigens, by supporting lysosomal protein degradation. (PubMed:15894275, PubMed:20518820) Related references: PMID:15894275, PMID:20518820
• Microbial infection: Interacts with both FURIN and the unprocessed form of the mumps virus fusion protein F, facilitating FURIN-mediated cleavage of the viral protein.
• Subcellular localization: Predominantly resides in the membranes of lysosomes, late endosomes, and autophagosomes; also cycles to the cell membrane.
• Tissue specificity: Isoform LAMP-2A is highly expressed in placenta, lung, and liver, while LAMP-2B is broadly distributed and LAMP-2C is prominent in heart, brain, and skeletal muscle. (PubMed:26856698, PubMed:7488019) Related references: PMID:26856698, PMID:7488019
• Key structural features include disulfide bonds, heavy N-linked glycosylation, a single transmembrane helix, and a short cytoplasmic tail. Genetic variants are linked to glycogen storage disease (Danon disease).

Experimental Guidance and Technical Tips

• For Western blot (WB), the predicted molecular weight is 45 kDa, but due to extensive glycosylation, LAMP2 typically migrates at approximately 100–120 kDa. Validate band identity using knockout or silencing controls.
• When performing immunohistochemistry (IHC-P), consider heat-induced antigen retrieval (e.g., citrate buffer, pH 6.0) to unmask the epitope and optimize staining.
• For ELISA, titrate the antibody and use a matched pair or direct detection to determine optimal working concentrations; verify specificity with recombinant LAMP2 protein.
• This antibody was validated using a synthetic peptide from the luminal domain (aa 311-410); it recognizes human, mouse, and rat LAMP2, making it suitable for cross-species studies.

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CamelBio offers diagnostic manufacturers and research institutions a one-stop sourcing platform for IVD raw materials, including validated monoclonal antibodies like this anti-LAMP2 clone. From bulk ancillary reagents to rare-target antibody pairs, we bridge your autophagy and lysosomal disease research with reliable supply and technical support.