KO Validated Anti-Laminin beta 1 Rabbit Polyclonal Antibody for WB, ELISA - P07942

Core Product Specifications and Parameters

Biological Background: Laminin beta 1 Function and Localization

• Laminin subunit beta-1 (Laminin B1 chain) is a major component of basement membranes, encoded by the LAMB1 gene. It assembles with alpha and gamma subunits to form laminin heterotrimers such as laminin-1, laminin-2, laminin-6, laminin-8, laminin-10, and laminin-12.
• Laminin beta-1 mediates cell attachment, migration, and organization into tissues during embryonic development by interacting with other extracellular matrix components.
• It plays a critical role in cerebral cortex development: required for integrity of the basement membrane/glia limitans, serving as an anchor for radial glial cell endfeet and a barrier to migrating neurons. Related references: PMID:23472759
• As a subunit of laminin-1 (laminin-111), it stimulates agrin-induced receptor clustering through a MuSK-independent pathway (By similarity).
• Subcellular localization: Secreted into the extracellular space and found in the basement membrane.
• Contains laminin EGF-like domains and a coiled-coil region; undergoes post-translational modifications including glycosylation and phosphorylation.
• Associated with disease: variants linked to lissencephaly; classified under keywords such as 'Disease variant' and 'Lissencephaly'.
• Molecular weight: approximately 198 kDa, consistent with the predicted mass of 198038 Da.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 1467-1786) of human Laminin beta 1, which may target the laminin EGF-like domain. This region is conserved between human and mouse, supporting the observed cross-reactivity.
• For Western blotting, consider using a low-percentage gel (e.g., 6-8%) and extended transfer times to accommodate the large protein size (198 kDa).
• Optimize antibody dilution and validate in relevant human or mouse tissue lysates known to express laminin beta-1, such as placenta, kidney, or brain.
• For ELISA, the antibody may be used as a capture or detection reagent; pairing with a compatible monoclonal antibody could enhance assay sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with efficient access to IVD raw materials. For extracellular matrix and basement membrane research, including laminin beta-1 studies, we offer validated antibodies, optimized reagent sets, and bulk ancillary supplies. Our capabilities extend to sourcing rare-target reagents, enabling seamless transition from assay concept to clinical application.