[KO Validated] Anti-Lamin A/C Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P02545

Core Product Specifications and Parameters

Biological Background: Lamin A/C Function and Localization

• Lamins are intermediate filament proteins that form the nuclear lamina, a fibrous meshwork underlying the inner nuclear membrane, providing structural support and organizing chromatin. Lamin A and lamin C, both encoded by the LMNA gene via alternative splicing, are major components of this structure and are present in approximately equal amounts. Related references: PMID:10080180 PMID:10580070 PMID:10587585

• The nuclear lamina acts as a scaffold for the nuclear envelope, anchoring chromatin and nuclear pore complexes, and plays a role in DNA replication, transcription, and nuclear assembly. Lamin A/C also contributes to nuclear mechanical stiffness and cellular mechanoresponse. Related references: PMID:23990565 PMID:25127216

• Cell cycle–dependent phosphorylation regulates lamina disassembly during mitosis and reassembly in telophase, ensuring proper nuclear envelope dynamics. Related references: PMID:2188730 PMID:2344612

• Lamin A/C participates in DNA double-strand break repair by stabilizing broken DNA ends and preventing chromosome translocation, interacting with repair factors such as XRCC4. Related references: PMID:31548606

• Lamin A/C is essential for peripheral nervous system and skeletal muscle development, muscle satellite cell proliferation, osteoblastogenesis, and bone formation. It also prevents fatty infiltration of muscle and bone marrow, maintaining tissue volume and strength. Related references: PMID:10080180 PMID:10814726 PMID:11799477 PMID:12075506 PMID:15317753 PMID:18611980 PMID:10587585

• In the cardiovascular system, prelamin-A/C accumulation in vascular smooth muscle cells (VSMCs) accelerates senescence, disrupts mitosis, and induces DNA damage, contributing to age-related vascular pathology. Lamin A/C is also necessary for cardiac homeostasis. Related references: PMID:20458013 PMID:10580070 PMID:12927431 PMID:18611980

• Subcellularly, lamin A/C is localized primarily to the nuclear lamina, but also found in the nucleoplasm, nuclear matrix, and nuclear speckles, reflecting its dynamic roles. In arteries, prelamin-A/C accumulates with age and disease, particularly in atherosclerotic lesions, colocalizing with senescent VSMCs. Expression increases due to oxidative stress–mediated downregulation of ZMPSTE24.

Experimental Guidance and Technical Tips

• The immunogen (aa 403–572 of human lamin A/C) targets a C-terminal region conserved between lamin A and lamin C, making this polyclonal antibody suitable for detecting both isoforms in human, mouse, and rat samples. The KO validation verifies specificity.
• For Western blotting, the expected molecular weight is ~74 kDa for lamin A; lamin C may appear around ~65 kDa. Consider loading adequate protein and using appropriate lysis conditions, as lamin solubility can be affected by ionic strength and detergent.
• In immunofluorescence and IHC, lamin A/C primarily stains the nuclear periphery (lamina). Permeabilization and fixation conditions should be optimized for the target cell type or tissue.
• For IP, the antibody may capture lamin A/C complexes; perform appropriate controls.
• As lamin A/C undergoes various post-translational modifications (e.g., phosphorylation, acetylation), treatment with inhibitors or induction of cell cycle arrest may alter detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a streamlined, one-stop channel for IVD raw material sourcing, technical services, and project consultation. We supply validated monoclonal and polyclonal antibodies like this anti-Lamin A/C pAb, along with bulk ancillary reagents and custom rare-target antibody development, supporting nuclear envelope and laminopathy studies from discovery to clinical assay development. Partner with CamelBio to bridge your diagnostic innovation from concept to clinic.