KO-Validated Anti-Lamin A/C Mouse Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P02545

Core Product Specifications and Parameters

Biological Background: Lamin A/C Function and Localization

• Lamin A and C, the main protein products of the LMNA gene, form the nuclear lamina meshwork that underlies the inner nuclear membrane, bridging the nuclear envelope and chromatin to regulate nuclear assembly, chromatin organization, and telomere dynamics. Related references: PMID:10080180 PMID:10580070 PMID:10587585
• Lamin A/C governs nuclear mechanical properties and matrix stiffness, thereby influencing cellular mechanotransduction. Related references: PMID:23990565 PMID:25127216
• The nuclear lamina undergoes cell cycle-dependent disassembly in prophase and reassembly in telophase. Related references: PMID:2188730 PMID:2344612
• Lamin A/C functions in DNA repair by stabilizing DNA double-strand breaks and preventing chromosomal translocations. Related references: PMID:31548606
• It is required for development and maintenance of the peripheral nervous system, skeletal muscle, bone, and heart, and prevents fat infiltration into muscle and bone marrow. Related references: PMID:10080180 PMID:10814726 PMID:11799477 PMID:12075506 PMID:15317753 PMID:18611980 PMID:10587585 PMID:10580070 PMID:12927431
• Prelamin-A/C accumulation in vascular smooth muscle cells promotes senescence by disrupting mitosis and causing DNA damage. Related references: PMID:20458013
• Lamin A/C localizes primarily to the nuclear lamina, nucleoplasm, and nuclear envelope; its accumulation in vascular smooth muscle cells increases with age and disease, correlating with cellular senescence and oxidative stress.
• LMNA mutations cause a spectrum of laminopathies, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy, limb-girdle muscular dystrophy, and Hutchinson-Gilford progeria syndrome, often linked to aberrant post-translational processing.

Experimental Guidance and Technical Tips

• Consider using nuclear-enriched lysates for WB to enhance lamin A/C detection; the antibody recognizes a ~74 kDa band.
• Use LMNA knockout cell lines as negative controls to confirm antibody specificity.
• For immunofluorescence, permeabilize cells with 0.1% Triton X-100 to visualize the nuclear lamina and expect rim staining.
• The immunogen spans amino acids 403–572, a region common to both lamin A and C, so the antibody likely detects both isoforms; verify in your experimental context.
• Pre-test on mouse and rat tissues/cells to validate cross-reactivity before large-scale experiments.
• For IHC-P, optimize antigen retrieval (e.g., citrate buffer) and include appropriate positive tissue controls (skeletal muscle, skin).
• For ELISA applications, titrate the antibody and optimize coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing solution for IVD raw materials, supplying diagnostic manufacturers and research laboratories with validated antibodies, antibody pairs, and ancillary reagents. The anti-Lamin A/C monoclonal antibody exemplifies our commitment to providing high-quality tools for nuclear lamina biology, laminopathy research, and aging studies. With a global network, CamelBio supports bulk procurement and custom sourcing for rare targets, ensuring a seamless supply chain from concept to clinic.