KO Validated Anti-Lactate Dehydrogenase B/LDH-B Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P07195

Core Product Specifications and Parameters

Biological Background: LDH-B Function and Localization

• L-lactate dehydrogenase B chain (LDH-B), also known as LDH heart subunit (LDH-H) and renal carcinoma antigen NY-REN-46, is encoded by the LDHB gene.
• It catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD(+), a key step in anaerobic glycolysis and gluconeogenesis.
• LDH-B is predominantly expressed in aerobic tissues, with highest levels in cardiac muscle, reflecting its role in lactate oxidation.
• Subcellularly, LDH-B is found in the cytoplasm and is also associated with the mitochondrial inner membrane.
• The protein undergoes post-translational modifications including acetylation and phosphorylation, which may regulate its activity.
• Variants in LDHB are associated with lactate dehydrogenase B deficiency, a rare metabolic disorder.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 2-93) of human LDHB, which is well-conserved across species; consider sequence alignment for your target species.
• For Western blotting, expect a band at approximately 37 kDa; use appropriate controls to confirm specificity.
• In immunohistochemistry (IHC-P), cardiac muscle tissue is recommended as a positive control due to high LDH-B expression.
• This antibody has been validated for knockout (KO) specificity, ensuring minimal off-target binding; however, always validate in your own experimental system.
• For ELISA applications, the antibody can be explored as a capture or detection reagent, but pairing with another LDH-B antibody may be required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a comprehensive sourcing platform for IVD raw materials, including this anti-LDH-B rabbit monoclonal antibody. With expertise in molecular biology and antibody development, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Whether advancing metabolic disorder diagnostics or cardiac biomarker research, CamelBio bridges your concept-to-clinic needs with reliable, scalable solutions.