KO Validated Anti-KRAS Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P01116

Core Product Specifications and Parameters

Biological Background: KRAS Function and Localization

• KRAS (GTPase KRas), also known as K-Ras 2, Ki-Ras, c-K-ras, and c-Ki-ras, is encoded by the KRAS (KRAS2, RASK2) gene. Multiple protein isoforms are generated through alternative splicing.
• It functions as a signal transducer in the Ras-MAPK signaling pathway, regulating cell proliferation and survival (PubMed:22711838, PubMed:23698361). Related references: PMID:22711838, PMID:23698361
• KRAS binds GDP/GTP and possesses intrinsic GTPase activity, cycling between active and inactive states (PubMed:20949621, PubMed:39809765). Related references: PMID:20949621, PMID:39809765
• In colorectal cancer cells, KRAS contributes to oncogenesis by inducing ZNF304-dependent transcriptional silencing of tumor suppressor genes (PubMed:24623306). Related references: PMID:24623306
• The protein is recognized by the LZTR1 adaptor, leading to its ubiquitination and degradation via a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex (PubMed:40934300). Related references: PMID:40934300
• Subcellularly, KRAS is associated with the cell membrane, the endomembrane system, and the cytoplasm.
• At the protein level, KRAS is expressed throughout the epidermis, with weak expression in the stratum corneum and dermis.
• Post-translational modifications include acetylation, methylation, prenylation, palmitoylation, and ubiquitination, which regulate its localization and function.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 30–188 within the conserved GTPase domain; cross-reactivity with mouse and rat is consistent with high sequence identity in this region. If testing additional species, align sequences beforehand.
• For Western blotting, expect a band around 22 kDa. Use KO-validated cell lysates as a negative control to confirm band specificity, and titrate the antibody for optimal signal-to-noise ratios in your system.
• In immunofluorescence/immunocytochemistry, KRAS localizes to membranes and cytoplasm; optimize fixation and permeabilization steps. Include KO cell lines to verify staining specificity.
• For ELISA, validate the antibody under your specific assay conditions, as performance can vary with coating and detection methods.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic developers from early R&D through clinical applications. This KRAS polyclonal antibody, targeting a pivotal oncogenic signal transducer, is well-suited for cancer biomarker research and assay development. In addition to individual antibodies, CamelBio provides validated antibody pairs, bulk ancillary reagents, and customized procurement for rare or difficult-to-obtain raw materials, streamlining your supply chain and accelerating product development.