KO Validated Anti-KMT5C Polyclonal Antibody for WB, ELISA - Q86Y97

Core Product Specifications and Parameters

Biological Background: KMT5C Function and Localization

• KMT5C (also known as SUV420H2) is a histone-lysine N-methyltransferase that specifically methylates monomethylated 'Lys-20' (H4K20me1) and dimethylated 'Lys-20' (H4K20me2) of histone H4 to produce di- and trimethylated forms, respectively; it also shows in vitro activity on unmethylated H4K20me0. Related references: PMID:24396869
• H4K20 trimethylation is a hallmark of transcriptional repression and constitutive heterochromatin formation, particularly at pericentric regions. KMT5C is targeted to histone H3 via interaction with RB1 family proteins (RB1, RBL1, RBL2).
• KMT5C facilitates TP53BP1 foci formation upon DNA damage and proficient non-homologous end-joining (NHEJ)-directed DNA repair by catalyzing the di- and trimethylation of H4K20. Related references: PMID:28114273
• Subcellular localization is primarily nuclear, associated with chromosomes.
• KMT5C is a phosphoprotein and a metal-binding methyltransferase that uses S-adenosyl-L-methionine as a cofactor.
• Alternative splicing generates multiple isoforms of KMT5C.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide within amino acids 100–200 of human KMT5C. This linear epitope design makes it well-suited for denaturing western blotting (WB) and peptide-based ELISA.
• For WB, a band at approximately 52 kDa is expected. Use appropriate gel percentage and validate specificity by comparing signals in wild-type versus KMT5C knockout cell lysates.
• Cross-reactivity is confirmed for human and mouse; perform species-specific validation for other organisms.
• As a polyclonal antibody, note that inter-batch consistency may vary; consider lot-specific validation in critical assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies this anti-KMT5C antibody as part of a comprehensive IVD raw-material portfolio for epigenetic and chromatin research. We provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing support to accelerate your assay development from concept to clinic.