[KO Validated] Anti-KEAP1 Rabbit Monoclonal Antibody (INrf2/KLHL19) - Q14145

Core Product Specifications and Parameters

Biological Background: KEAP1 Function and Localization

• KEAP1 (Kelch-like ECH-associated protein 1), also known as INrf2 or KLHL19, is a substrate-specific adapter for the BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex.
• It regulates oxidative stress response by targeting the transcription factor NFE2L2/NRF2 for ubiquitination and proteasomal degradation under normal conditions. Related references: PMID:14585973 PMID:15379550 PMID:15572695
• Oxidative or electrophilic stress triggers covalent modification of reactive cysteine residues in KEAP1, inactivating the ligase and allowing NRF2 to accumulate and activate cytoprotective genes. Related references: PMID:16006525 PMID:17127771 PMID:18251510
• KEAP1 also mediates ubiquitination of substrates like SQSTM1/p62, BPTF, and PGAM5, and is involved in selective autophagy pathways. Related references: PMID:15379550 PMID:17046835 PMID:20452972 PMID:28380357
• Localizes to both cytoplasm and nucleus.
• Broadly expressed, with highest levels in skeletal muscle.
• Contains Kelch repeats and a BTB domain; classified under host-virus interaction, S-nitrosylation, and Ubl conjugation pathways.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 525-624) of human KEAP1, which includes Kelch repeat domains critical for substrate binding. This region is well conserved, supporting cross-reactivity with mouse and rat.
• For Western blotting, a band around 70 kDa is expected. Consider that post-translational modifications (e.g., ubiquitination) or oxidative modifications may alter migration slightly.
• For IF/ICC, both cytoplasmic and nuclear staining may be observed depending on cellular stress conditions.
• The antibody has been KO validated, confirming specificity; however, always validate in your specific sample system and include appropriate controls.
• For ELISA, direct binding to the immunogen peptide should be confirmed; pairing with a capture antibody may require optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to IVD raw materials, including validated monoclonal antibodies like this anti-KEAP1 rabbit mAb. We offer bulk monoclonal/polyclonal antibodies, ancillary reagents, and technical consulting for projects focused on oxidative stress and the NRF2 signaling pathway. Contact us to explore customized sourcing solutions and accelerate your assay development.