KO Validated Anti-JAK2 Rabbit Polyclonal Antibody for WB, ELISA - O60674

Core Product Specifications and Parameters

Biological Background: JAK2 Function and Localization

• JAK2 is a non-membrane spanning protein tyrosine kinase that phosphorylates a wide array of type I receptors (e.g., growth hormone, prolactin, leptin, erythropoietin, thrombopoietin receptors) and type II receptors (e.g., interferon-alpha/beta/gamma, interleukin receptors), initiating diverse signaling cascades. Related references: PMID:15690087 PMID:15899890 PMID:7615558
• Upon receptor activation, JAK2 phosphorylates cytoplasmic tyrosine residues to create docking sites for STAT transcription factors, which upon phosphorylation, dimerize, and shuttle to the nucleus to regulate gene expression. Related references: PMID:15690087 PMID:9618263
• In erythropoiesis, EPO binding triggers JAK2 autophosphorylation and activation, leading to EPOR phosphorylation and subsequent STAT5-mediated transcription of erythroid-specific genes. Related references: PMID:9657743
• Within the nucleus, JAK2 phosphorylates histone H3 at tyrosine 41 (H3Y41ph), ejecting HP1α from chromatin and thereby modulating epigenetic states in response to cytokine signals. Related references: PMID:19783980
• JAK2 also regulates cell cycle progression by phosphorylating CDKN1B and modulates ion channel activity via KCNA3 phosphorylation. Related references: PMID:21423214 PMID:25644777
• Ubiquitously expressed across tissues, JAK2 localizes to the cytoplasm, nucleus, and endomembrane system, underscoring its multifaceted signaling roles.
• Classified as a proto-oncogene, chromatin regulator, and pivotal kinase in innate and adaptive immunity, with disease-associated variants linked to myeloproliferative disorders.

Experimental Guidance and Technical Tips

• The immunogen spans the kinase domain (aa 933–1132); thus, the antibody may detect both full-length and truncated JAK2 forms in denaturing Western blot.
• For WB, expect a specific band near 131 kDa; using JAK2 knockout lysates is recommended to confirm band identity.
• In ELISA, the polyclonal nature can enhance detection sensitivity; however, pairing with a matched monoclonal antibody may improve assay reproducibility.
• While cross-reactivity with mouse and rat is indicated, verify target sequence homology in your specific model system.
• As a rabbit polyclonal antibody, lot-to-lot variability should be evaluated; consider batch consistency testing when integrating into diagnostic workflows.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes one-stop access to IVD raw materials, technical services, and consulting from concept to clinic. For JAK2‑related signal transduction and chromatin studies, we provide high‑quality polyclonal antibodies, validated antibody pairs, and bulk ancillary reagents. Our sourcing expertise extends to rare‑target raw materials, supporting your entire assay development pipeline.