KO Validated Anti-ITCH Polyclonal Antibody for WB, ELISA - Q96J02

Core Product Specifications and Parameters

Biological Background: ITCH Function and Localization

• ITCH is an E3 ubiquitin-protein ligase that accepts ubiquitin from E2 enzymes and directly transfers it to substrates, catalyzing Lys-29, Lys-48, and Lys-63-linked polyubiquitin chains to regulate protein stability, trafficking, and signaling. Related references: PMID:11046148 PMID:14602072 PMID:15051726 PMID:17028573 PMID:18718448 PMID:19131965
• As a core component of a ubiquitin-editing complex with TNFAIP3, TAX1BP1, and RNF11, ITCH promotes Lys-63 to Lys-48 ubiquitin switching on RIPK1, leading to its proteasomal degradation and termination of TNF/NF-κB inflammatory signaling. Related references: PMID:19131965
• ITCH ubiquitinates the innate immune kinase RIPK2 via Lys-63-linked chains to modulate NOD2 signaling, and targets the mitochondrial antiviral protein MAVS for Lys-48-mediated degradation, thus negatively regulating antiviral responses. Related references: PMID:19592251 PMID:19881509
• In hematopoietic cells, ITCH ubiquitinates transcription factor NFE2 with Lys-63 linkages, controlling hematopoietic lineage development, and drives JUNB ubiquitination and degradation to limit Th2 cytokine production. Related references: PMID:18718448 PMID:16387660
• ITCH regulates endosomal sorting of receptors such as FZD4 and CXCR4 via ubiquitination of PI4K2A or ESCRT-0 components HGS and STAM, linking ligand stimulation to degradative endocytic pathways. Related references: PMID:23146885 PMID:14602072
• The ligase promotes apoptosis through ubiquitination and degradation of pro-apoptotic factors p15 BID and TXNIP (in collaboration with UBR5), and mediates the anti-apoptotic effect of EGFR signaling. Related references: PMID:20392206 PMID:29378950
• Subcellularly, ITCH is found at the cell membrane, cytoplasm, nucleus, and on early endosome and endosome membranes, consistent with its diverse functional roles.
• ITCH is widely expressed in human tissues and is post-translationally modified by acetylation and phosphorylation, which may regulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–155 of human ITCH (NP_001244066.1). This N-terminal region may allow detection of full-length ITCH and potential N-terminal-containing splice variants, but experimental verification in the relevant sample system is recommended.
• For Western blotting, a band near the predicted molecular weight of 103 kDa is expected. Use appropriate controls (e.g., ITCH knockout lysates or siRNA) to confirm specificity, especially given ITCH’s role as a large, multi-domain protein.
• Cross-reactivity is indicated for human, mouse, and rat samples. Validate the antibody in your specific species and tissue context, as expression levels and post-translational modifications may affect recognition.
• The antibody is suitable for ELISA; optimize capture/detection conditions using recombinant ITCH protein or cell lysates, and pair with a compatible detection system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, offering validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare target procurement. Our ITCH-related offerings support research into ubiquitin signaling, inflammation, apoptosis, and immune regulation, empowering diagnostic and therapeutic development from concept to clinic.