[KO Validated] Anti-IRF9 Polyclonal Antibody for WB, ELISA - Q00978

Core Product Specifications and Parameters

Biological Background: IRF9 Function and Localization

• IRF9 (Interferon regulatory factor 9), also known as ISGF3γ or p48, is a key transcription factor in the type I interferon (IFN-α/β) signaling pathway.
• Upon type I IFN binding to cell surface receptors, kinases TYK2 and JAK1 are activated, leading to tyrosine phosphorylation of STAT1 and STAT2.
• IRF9 associates with phosphorylated STAT1:STAT2 dimer to form the ISGF3 complex, which translocates to the nucleus.
• In the nucleus, ISGF3 binds to the interferon-stimulated response element (ISRE) to drive transcription of interferon-stimulated genes (ISGs), inducing an antiviral state.
• IRF9 belongs to the interferon regulatory factor family and is critical for innate antiviral immunity.
• Subcellularly, IRF9 shuttles between the cytoplasm and nucleus; its nuclear accumulation is enhanced upon IFN signaling.
• Post-translational modifications include phosphorylation and ubiquitin-like conjugation, which may regulate its activity and stability.

Experimental Guidance and Technical Tips

• The antibody is identified as KO-validated by the supplier; use this feature to confirm specificity in your own experimental setup.
• For Western blot, a band around 44 kDa is expected; include both positive (e.g., IFN-treated human cells) and negative controls.
• In ELISA, optimize coating conditions and antibody concentrations for your specific assay format.
• Being a polyclonal preparation, lot-to-lot consistency should be verified if used in quantitative or diagnostic applications.
• The immunogen covers amino acids 113–255, so the antibody may recognize multiple IRF9 isoforms sharing this epitope; verify in your sample context.
• When using out-of-species from the validated human cross-reactivity, perform preliminary testing to confirm reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw-material sourcing, from validated antibody pairs to bulk ancillary reagents. This anti-IRF9 polyclonal antibody supports research into interferon signaling and innate immunity, critical areas for diagnostic development. Trust our integrated supply chain to deliver consistent, high-quality materials for your projects, from concept to clinic.