[KO Validated] Anti-IRF3 Rabbit Monoclonal Antibody for WB - Q14653

Core Product Specifications and Parameters

Biological Background: IRF3 Function and Localization

• IRF3 is a key transcriptional regulator of type I interferon (IFN)-dependent immune responses, playing a critical role in the innate immune defense against DNA and RNA viruses. Related references: PMID:22394562 PMID:24049179 PMID:25636800
• In uninfected cells, IRF3 resides in the cytoplasm in an inactive form. Upon viral infection or toll-like receptor (TLR) signaling, it becomes phosphorylated by IKBKE and TBK1 kinases, triggering dimerization and nuclear translocation. Related references: PMID:22394562 PMID:25636800 PMID:27302953
• Activated IRF3 associates with CREB binding protein (CREBBP) to form the dsRNA-activated factor 1 (DRAF1) complex, which binds to interferon-stimulated response elements (ISRE) and drives transcription of type I IFN and interferon-stimulated genes (ISGs). Related references: PMID:16154084 PMID:27302953 PMID:33440148
• IRF3 can be recruited to mitochondria via TOMM70:HSP90AA1, forming an apoptosis complex that induces programmed cell death in response to certain viral infections. Related references: PMID:25609812
• Subcellular localization includes cytoplasm, nucleus, and mitochondrion. IRF3 shuttles between compartments depending on its activation status.
• Constitutively expressed across a variety of tissues, ensuring broad antiviral surveillance.
• Post-translational modifications such as phosphorylation, acetylation, and ubiquitination regulate IRF3 activity, stability, and interaction with other proteins.
• Alternative splicing generates multiple isoforms, potentially modulating IRF3 function in different cellular contexts.

Experimental Guidance and Technical Tips

• The antibody targets an epitope within amino acids 50–150 of human IRF3; verify specificity using the KO validated product line or by knockdown/knockout in your experimental system.
• For Western blot, expect a band at approximately 47 kDa. Use positive controls such as virus-infected or poly(I:C)-stimulated cell lysates to enhance detection of endogenous IRF3.
• In ELISA applications, consider testing the antibody as capture or detection reagent in combination with a matched IRF3 antibody pair, and validate with recombinant IRF3 protein.
• Because IRF3 functions in innate immunity, activate pathways with ligands like poly(I:C) or Sendai virus to study phosphorylation and translocation in human or mouse models.

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