[KO Validated] Anti-IPO4 Rabbit Polyclonal Antibody for WB, ELISA - Q8TEX9

Core Product Specifications and Parameters

Biological Background: IPO4 Function and Localization

• IPO4 (Importin-4), also known as Imp4b or RanBP4, is a nuclear transport receptor belonging to the importin-β family.
• It functions as a receptor for nuclear localization signals (NLS), mediating the nuclear import of diverse cargo proteins including histones, RPS3A, TNP2, and VDR in a Ran-dependent manner. Related references: PMID:11823430, PMID:16207705, PMID:17682055
• IPO4 specifically imports histone H3-H4 dimers when they are in complex with ASF1 chaperones, a critical step for chromatin assembly. Related references: PMID:21454524, PMID:29408485, PMID:36103578
• It also transports monomeric histones H3.1 and H4 into the nucleus. Related references: PMID:30177573
• IPO4 mediates the ligand-independent nuclear import of the vitamin D receptor (VDR), influencing transcriptional regulation. Related references: PMID:16207705
• In vitro, IPO4 can recognize a non-classical NLS on human cytomegalovirus UL84 protein to facilitate its nuclear entry. Related references: PMID:12610148
• Subcellularly, IPO4 shuttles between the cytoplasm and nucleus, consistent with its transport role.
• The protein is subject to acetylation and alternative splicing, and it contains HEAT repeats characteristic of importin-β family members.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 728–1081) of human IPO4; consider verifying that the antibody recognizes epitopes in this domain by testing in relevant cell or tissue lysates.
• For Western blotting, IPO4 is predicted at ~119 kDa; use an appropriate gel percentage and transfer conditions to ensure clear detection.
• The antibody shows cross-reactivity with mouse and rat IPO4; validate specificity and signal in your model system using positive and negative controls.
• KO validation suggests the antibody can be used for knockout/knockdown confirmation; include appropriate lysates from wild-type and IPO4-deficient samples when interpreting results.
• For ELISA applications, optimization of coating antigen and detection conditions is recommended to achieve optimal signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers streamlined access to a comprehensive portfolio of IVD raw materials, from validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents. Our expertise in nuclear transport and chromatin biology targets like IPO4 enables us to support diagnostic developers with rare-target sourcing and tailored supply solutions. Partner with CamelBio to bridge the gap from concept to clinic with reliable, high-quality immunoreagents.