KO Validated Anti-Insulin-Degrading Enzyme (IDE) Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P14735

Core Product Specifications and Parameters

Biological Background: Insulin-degrading enzyme (IDE) Function and Localization

• IDE is a zinc metalloprotease (insulysin/Abeta-degrading protease) that degrades insulin, glucagon, IAPP, and natriuretic peptides, regulating glucose homeostasis and peptide signaling (PubMed:10684867, PubMed:17051221, PubMed:17613531, PubMed:21098034). Related references: PMID:10684867, PMID:17051221, PMID:17613531, PMID:21098034
• Substrate binding triggers conformational changes, enabling IDE to process structurally diverse large substrates like insulin (PubMed:23922390, PubMed:26394692, PubMed:29596046). Related references: PMID:23922390, PMID:26394692, PMID:29596046
• IDE clears APP-derived amyloidogenic peptides, including Abeta, and thus impacts Alzheimer’s disease pathology (PubMed:26394692, PubMed:9830016). Related references: PMID:26394692, PMID:9830016
• It processes peptide hormones such as bradykinin and kallidin, and contributes to antigen presentation by generating MHC class I‑restricted epitopes.
• Localizes to the cytoplasm and is secreted; a membrane‑associated isoform acts as an entry receptor for varicella‑zoster virus.
• Highly expressed in brain and detectable in cerebrospinal fluid, aligning with its role in neural peptide clearance.
• Allosteric regulation and ATP binding are notable features; the enzyme requires zinc for catalytic activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1‑250 of human IDE, so the antibody may recognize both full-length and N‑terminal fragments.
• For WB, a band at ~118 kDa is expected; validate specificity using appropriate positive (e.g., HeLa, brain lysate) and negative controls.
• In IF/ICC and IHC-P, consider testing neuronal cell lines or brain tissue sections where IDE is abundantly expressed.
• Cross‑reactivity with mouse IDE makes this antibody suitable for studies in transgenic mouse models; however, confirm cross‑reactivity in your specific assay system.
• For ELISA, pair with a capture/detection system optimized for rabbit polyclonal antibodies and consider using recombinant IDE as a standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with one-stop access to high-quality IVD raw materials, including validated antibodies and ancillary reagents. This anti-IDE polyclonal antibody supports research into diabetes, neurodegeneration, and peptide hormone regulation, and can be incorporated into assay development workflows. For bulk orders or custom sourcing of rare-target antibodies and disease‑associated biomarker reagents, contact CamelBio’s supply team.