[KO Validated] Anti-ING5 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8WYH8

Core Product Specifications and Parameters

Biological Background: ING5 Function and Localization

• ING5, also known as p28ING5, is an inhibitor of growth protein that functions as a component of histone acetyltransferase complexes.
• It is a core subunit of the HBO1 complex, which acetylates histone H3 at Lys-14 (H3K14ac) and histone H4. Related references: PMID:24065767
• ING5 also associates with the MOZ/MORF complex, contributing to its histone H3 acetyltransferase activity. Related references: PMID:16387653
• Through chromatin acetylation, ING5 may regulate DNA replication and act as a transcriptional coactivator. Related references: PMID:12750254 PMID:16387653
• ING5 inhibits cell growth, delays S-phase progression, and enhances Fas-induced apoptosis in an INCA1-dependent manner. Related references: PMID:21750715
• The protein localizes to the nucleus and is associated with chromosomes, consistent with its role in chromatin modification.
• ING5 is subject to post‑translational modifications including acetylation, methylation, and phosphorylation, and contains a zinc‑finger domain.
• In acute myeloid leukemia, ING5 is down‑regulated in bone marrow cells compared to normal bone marrow.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal 1‑220 amino acids of human ING5; verify epitope accessibility in your assay format.
• For Western blot, consider starting with a dilution range of 1:500–1:2000 using nuclear extracts or whole‑cell lysates from human cell lines.
• For IF/ICC, permeabilize cells to allow nuclear access and include a nuclear counterstain to confirm localization.
• Validate antibody specificity in your system using appropriate positive and negative controls (e.g., ING5 knockout cell lines).

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio is a dedicated IVD raw‑material partner offering diagnostic manufacturers, laboratories, and research institutes streamlined access to validated antibodies and ancillary reagents. For chromatin biology and oncology projects, we supply high‑quality polyclonal and monoclonal antibodies against key histone modifiers like ING5, along with bulk sourcing and personalized technical support—bridging the gap from early‑stage research to clinical application.